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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-48076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHST4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48076) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHST4 in SW13 human adrenal cortex adenocarcinoma cell line. Samples were incubated in CHST4 polyclonal antibody (Product # PA5-48076) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
