Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MA1-10183 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3e Monoclonal Antibody (APA1/1), PE
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- APA1/1
- Vial size
- 100 µg
- Concentration
- 0.1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of Jurkat cells stained using anti-CD3 epsilon activation epitope (APA1/1) PE Monoclonal antibody (Product # MA1-10183) (concentration in sample 3 µg/mL, red) from unstained Jurkat cells (grey) in flow cytometry analysis (intracellular staining).
- Conjugate
- Yellow dye