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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunocytochemistry [2]
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- Product number
- MA5-17043 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (4D10A6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17043 targets CD3 in indirect ELISA, ICC and IF applications and shows reactivity with Human samples. The MA5-17043 immunogen is purified recombinant fragment of human CD3 expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D10A6
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of peripheral blood T cells using CD3e/TCRE monoclonal antibody (Product # MA5-17043) (A). Flow cytometric analysis of eripheral blood T cells using CD3e/TCRE monoclonal antibody (Product # MA5-17043) (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD3 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD3 Monoclonal Antibody (4D10A6) (Product # MA5-17043) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents Raji showing no expression of CD3. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
