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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NBP2-37709 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD3 zeta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Antibody recognizes phosphorylated tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta [p tyr 111] Antibody (EM-55) [NBP2-37709] - Analysis in lysates of HEK293T/17 cells transiently transfected with expression vector harboring gene for mCD3zeta. Lane 1) Unstimulated cells, Lane 2) Pervanadate-stimulated cells
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).
