Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP2-37708 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD3 zeta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta [p Tyr 142] Antibody (EM-54) [NBP2-37708] - Analysis of antibody in lysates of HEK293T/17 cells transiently transfected with expression vector harboring gene for mCD3zeta. Lane 1) Unstimulated cells, Lane 2) Pervanadate-stimulated cells
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5