644105

antibody from BioLegend

Targeting: CD247 CD3H, CD3Q, CD3Z

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

644105

Provider

BioLegend

Proper citation

BioLegend Cat#644105, RRID:AB_2565720

Product name

PE anti-CD247 (TCRζ, CD3ζ)

Antibody type

Monoclonal

Antigen

Human TCR zeta chain aa38-54

Description

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

6B10.2

Vial size

25 µg

Concentration

0.2 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).

Conjugate

Yellow dye