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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG65443 - Provider product page
- Provider
- Arigo
- Product name
- anti-CD3 zeta phospho (Tyr72) antibody [EM-26]
- Antibody type
- Monoclonal
- Antigen
- A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
- Description
- Purified from cell culture supernatant by protein-A affinity chromatography.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EM-26
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at 2-8°C. Do not freeze. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Cell lysates with various phosphorylated particular human CD3 zeta mutants stained with antibody clone EM-26 Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Immunological synapse formed between the lymph node naive T cells from TCR transgenic mice and DCEK cells treated with MCC peptide for 20 min stained with clone EM-26 (light blue) Actin cytoskeleton (red).
