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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ARG65722 - Provider product page
- Provider
- Arigo
- Product name
- anti-CD3 zeta phospho (Tyr142) antibody [EM-54]
- Antibody type
- Monoclonal
- Antigen
- Phosphospecific peptide around Tyr142 of Human CD3 zeta.
- Description
- Purification with Protein A.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EM-54
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- Arigo (provider)
- Main image
- Experimental details
- Westren blot: Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were stained with ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54]. Lanes:1) Wt mCD3zeta pervanadate non-stimulated2) Wt mCD3zeta pervanadate stimulated3) mut. mCD3zeta Y/F2-64) mut. mCD3zeta Y/F1 and 3-65) mut. mCD3zeta Y/F1-2 and 4-66) mut. mCD3zeta Y/F1-3 and 5-67) mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)8) mut. mCD3zeta Y/F1-5.
