H00000919-D01P

antibody from Abnova Corporation

Targeting: CD247 CD3H, CD3Q, CD3Z

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000919-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000919-D01P, RRID:AB_1673170

Product name

CD247 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CD247 protein.

Antigen sequence

MKWKALFTAAILQAQLPITEAQSFGLLDPKLCYLL
DGILFIYGVILTALFLRVKFSRSADAPAYQQGQNQ
LYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKN
PQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDG
LYQGLSTATKDTYDALHMQALPPR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CD247 expression in transfected 293T cell line (H00000919-T01) by CD247 MaxPab polyclonal antibody.Lane 1: CD247 transfected lysate(18.70 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CD247 MaxPab rabbit polyclonal antibody. Western Blot analysis of CD247 expression in IMR-32.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CD247 MaxPab rabbit polyclonal antibody. Western Blot analysis of CD247 expression in rat brain.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CD247 MaxPab rabbit polyclonal antibody. Western Blot analysis of CD247 expression in Raw 264.7.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CD247 MaxPab rabbit polyclonal antibody. Western Blot analysis of CD247 expression in mouse kidney.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CD247 and CD3E. HeLa cells were stained with anti-CD247 rabbit purified polyclonal 1:1200 and anti-CD3E mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)