Antibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Flow cytometry [1]
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- Product number
- 11-0036-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (SK7), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitopes.
- Conjugate
- Green dye
- Antibody clone number
- SK7
- Concentration
- 5 µL/Test
Submitted references Heterozygous premature termination in zinc-finger domain of Krüppel-like factor 2 gene associates with dysregulated immunity.
A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.
Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection.
Human oral mucosa cell atlas reveals a stromal-neutrophil axis regulating tissue immunity.
Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer.
HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.
Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity.
Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.
Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Mutant ASXL1 cooperates with BAP1 to promote myeloid leukaemogenesis.
Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.
Pernaa N, Keskitalo S, Chowdhury I, Nissinen A, Glumoff V, Keski-Filppula R, Junttila J, Eklund KK, Santaniemi W, Siitonen S, Seppänen MR, Vähäsalo P, Varjosalo M, Åström P, Hautala T
Frontiers in immunology 2022;13:819929
Frontiers in immunology 2022;13:819929
A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.
Staels F, Lorenzetti F, De Keukeleere K, Willemsen M, Gerbaux M, Neumann J, Tousseyn T, Pasciuto E, De Munter P, Bossuyt X, Gijsbers R, Liston A, Humblet-Baron S, Schrijvers R
Journal of clinical immunology 2022 Nov;42(8):1638-1652
Journal of clinical immunology 2022 Nov;42(8):1638-1652
Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection.
Kenney DJ, O'Connell AK, Turcinovic J, Montanaro P, Hekman RM, Tamura T, Berneshawi AR, Cafiero TR, Al Abdullatif S, Blum B, Goldstein SI, Heller BL, Gertje HP, Bullitt E, Trachtenberg AJ, Chavez E, Nono ET, Morrison C, Tseng AE, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber BR, Saeed M, Balazs AB, Francis KP, Klose A, Paragas N, Campbell JD, Connor JH, Emili A, Crossland NA, Ploss A, Douam F
Cell reports 2022 Apr 19;39(3):110714
Cell reports 2022 Apr 19;39(3):110714
Human oral mucosa cell atlas reveals a stromal-neutrophil axis regulating tissue immunity.
Williams DW, Greenwell-Wild T, Brenchley L, Dutzan N, Overmiller A, Sawaya AP, Webb S, Martin D, NIDCD/NIDCR Genomics and Computational Biology Core, Hajishengallis G, Divaris K, Morasso M, Haniffa M, Moutsopoulos NM
Cell 2021 Jul 22;184(15):4090-4104.e15
Cell 2021 Jul 22;184(15):4090-4104.e15
Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer.
Wang C, Weng M, Xia S, Zhang M, Chen C, Tang J, Huang D, Yu H, Sun W, Zhang H, Lai M
Cancer science 2021 Jan;112(1):178-193
Cancer science 2021 Jan;112(1):178-193
HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.
Kim YD, Park SM, Ha HC, Lee AR, Won H, Cha H, Cho S, Cho JM
Journal of Cancer 2020;11(14):4059-4072
Journal of Cancer 2020;11(14):4059-4072
Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity.
Stunnenberg M, Sprokholt JK, van Hamme JL, Kaptein TM, Zijlstra-Willems EM, Gringhuis SI, Geijtenbeek TBH
Frontiers in immunology 2020;11:8
Frontiers in immunology 2020;11:8
Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.
Zou Y, Liang J, Li D, Fang J, Wang L, Wang J, Zhang J, Guo Q, Yan X, Tang H
Oncology letters 2020 Jul;20(1):123-134
Oncology letters 2020 Jul;20(1):123-134
Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.
Choi JW, Lee ES, Kim SY, Park SI, Oh S, Kang JH, Ryu HA, Lee S
BMC cancer 2019 Aug 19;19(1):817
BMC cancer 2019 Aug 19;19(1):817
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
Mutant ASXL1 cooperates with BAP1 to promote myeloid leukaemogenesis.
Asada S, Goyama S, Inoue D, Shikata S, Takeda R, Fukushima T, Yonezawa T, Fujino T, Hayashi Y, Kawabata KC, Fukuyama T, Tanaka Y, Yokoyama A, Yamazaki S, Kozuka-Hata H, Oyama M, Kojima S, Kawazu M, Mano H, Kitamura T
Nature communications 2018 Jul 16;9(1):2733
Nature communications 2018 Jul 16;9(1):2733
Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.
Yunusbaeva M, Valiev R, Bilalov F, Sultanova Z, Sharipova L, Yunusbayev B
Scientific reports 2018 Apr 17;8(1):6098
Scientific reports 2018 Apr 17;8(1):6098
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
Zhang W, Nie L, Wang Y, Wang XP, Zhao H, Dongol S, Maharjan S, Cheng L
PloS one 2013;8(6):e66286
PloS one 2013;8(6):e66286
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.
Senechal B, Elain G, Jeziorski E, Grondin V, Patey-Mariaud de Serre N, Jaubert F, Beldjord K, Lellouch A, Glorion C, Zerah M, Mary P, Barkaoui M, Emile JF, Boccon-Gibod L, Josset P, Debré M, Fischer A, Donadieu J, Geissmann F
PLoS medicine 2007 Aug;4(8):e253
PLoS medicine 2007 Aug;4(8):e253
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD3 FITC (right). Cells in the lymphocyte gate were used for analysis.
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- Green dye
Supportive validation
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- Figure 7 Circulating percentages of Th17 cells and CCR6-positive cells in peripheral blood are increased in IVD degenerated patients when compared with controls. Heparinized peripheral whole blood cells from 20 patients and 15 healthy controls were stimulated with phorbol myristate acetate (PMA), ionomycin, and monensin for 4 h and subsequently stained with fluorochrome-labeled antibodies as described in Materials and Methods. A(a) Lymphocytes were gated by flow cytometry. A(b) CD3 + T subsets were gated by flow cytometry; the plots in the inset box represent the CD3 + T cells. A(c) Representative IL-17 expression levels in the CD3 + CD8 - T subsets (CD4 + T subsets) from each group are shown. The percentages of positive cells are shown in the upper left panels. A(d) Representative surface CCR6 expression levels on the CD3 + CD8 - IL-17 + subsets from each group are shown. The percentages of positive cells are shown in the right panel. (B) The percentage of circulating Th17 cells was significantly higher in IVD degenerated patients (2.973+-0.689%) than in the control group (1.039+-0.156%; *, p
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- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
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- Figure 1 In vitro T cell proliferation. Mononuclear cells from psoriatic patients (red curve) and healthy controls (blue curve) were labelled with 1 uM CFSE prior to culturing and incubated for 5 days alone, with 1 ug/ml PHA ( A ) or in the presence of recombinant proteins K17 ( B ), S1 ( C ), S4 ( D ), PS1 ( E ) and PK ( F ) at concentration of 10 ug/ml. After 5 days, cells were labelled with a PE-conjugated anti-CD3 antibody and 7-AAD prior to flow cytometry analysis. Gated CD3+ lymphocytes are shown on the CFSE fluorescence histograms to demonstrate the decrease in fluorescence intensity during divisions. The higher peak for both patients and healthy controls represents a larger population of non-dividing parental cells (P). The signal of interest is the smaller peak that corresponds to dividing cells (shown using arrow under F ), and such cells are only observed in patents when treated with K17 ( B ), S1 ( C ), or S4 ( D ) proteins. Figures represent the percentage of CFSE low CD3+ cells (proliferating population).
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- Green dye
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- FIGURE 2 Expression of PD-L1 on human HCC PLC/PRF/5 cells. (A) Human HCC PLC/PRF/5 cells were cultured alone in the absence of GPC3-CAR T cells in RPMI 1640 medium containing 10% FBS. (B) Human HCC PLC/PRF/5 cells were cocultured with GPC3-CAR T cells at an effector:Target ratio of 1:1 for 18 h in RPMI 1640 medium containing 10% FBS. PD-L1 was determined by flow cytometry in the CD3-negative gate, and the fixable, viable stain 780 was used for discriminating live from dead cells.
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- FIGURE 4 Efficient disruption of PD-1 expression on the surface of GPC3-CAR T cells. PD-1 and CAR expression on the surface of T cells were detected by flow cytometry on day 3 after the re-stimulation with anti-CD3/anti-CD28 beads. UTD, untransduced T cells; WT, wild type.
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- Green dye
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- Fig. 2 Identification of key immune cell types of MYJ1633 following ex vivo expansion. a The distribution of NK cells (CD3 - CD16 + CD56 + ), NKT cells (CD3 + CD16 + CD56 + ), and T cells (CD3 + CD16 - CD56 - ) of freshly isolated PBMCs and MYJ1633 was examined by flow cytometry. b Proportion of helper T cells (Th cells; CD4 + ) and cytotoxic T cells (Tc cells; CD8 + ) among CD3 + cells of MYJ1633. These data were analyzed from 6 individuals (Additional file 1 : Figure S1). Significant differences between groups were determined by Student's t test. The data represented as mean +- SEM
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- Figure 6 HIV-1 Cap-RNA 58 -treated DCs mediate T cell proliferation and differentiation. DCs were unstimulated or treated with 1 nM HIV-1 Cap-RNA 58 or LPS for 48 h, cocultured with CellTrace Violet-labeled peripheral blood lymphocytes and harvested after 5 days. Proliferation was determined in (A) live CD3 + CD4 + cells and (B) CD3 + CD8 + T cells by flow cytometry. (C,D) DCs were treated with vehicle control, 1 nM HIV-1 control RNA, 1 nM HIV-1 Cap-RNA 58 , LPS, LPS + PGE 2 , or LPS + IFNgamma for 48 h and cocultured with naive T cells. After 11-13 days, cells were restimulated and intracellular expression levels of IFNgamma (T H 1) and IL-4 (T H 2) were analyzed using flow cytometry. Number in plots indicate percentage of cells in the quadrant. Data are representative of collated data of three (A,B) or four (D) donors, or four donors (C) of different experiments (mean +- s.d.). * P < 0.05, ** P < 0.01, Student's t -test. NS, not significant.
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- Figure 1 Proliferating Cells in LCH Granuloma are Mostly Endothelial Cells, Fibroblasts, and T Cells Paraffin-embedded and frozen sections were stained with antibodies against Ki-67 (which label proliferating cells), CD1a (LCs), CD3 (T cells), CD20 (B cells), CD68, CD31, and CD34 (endothelial cells). (A) Double immunostaining of paraffin-embedded section from LCH eosinophilic granulomas with anti-Ki-67 Ab, (brown nuclear staining) and with anti-CD1a Ab (upper images, blue staining) or anti-CD3 Ab (lower images, blue staining). Open arrowheads indicate double-stained cells, black arrowheads indicate Ki-67 + cells with an endothelial morphology. (B) Histogram represents percentage of CD1a + cells and of CD3 + cells labeled with Ki-67 ( n = 15). (C) Histogram represents percentage of proliferating cells (Ki-67 + ) that express CD1a, CD3, CD20, or CD68 ( n = 15). (D) Histogram represents percentage of proliferating cells (Ki-67 + ) that are endothelial cells, interstitial cells (fibroblasts), and other types based on morphological examination. (E) Immunolabeling of blood vessels on paraffin-embedded section from LCH eosinophilic granulomas with CD34 (left) and CD31 (right) antibodies. (F) Proliferating Ki-67 + cells (brown nuclear staining) with a fibroblast-cell morphology in an eosinophilic granuloma.
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- Figure 3 CG-745 increases helper T cells, cytotoxic T cells and natural killer T cells, and decreases Treg: (A) hPBMCs were incubated with CG (CG-745) for 36 hours and a subset of hPBMCs was analyzed using the antibodies indicated in the text; (B) hPBMCs were co-cultured with Huh7, Hep3B, HepG2 or PLC/PRF/5 cells for 36 hours with or without CG, and a subset of hPBMCs was analyzed by Attune Nxt (Invitrogen, USA).
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- Green dye