Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- 11-0039-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD3 Monoclonal Antibody (HIT3a), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the ? chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HIT3a
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ.
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Protective effects of SIRT1 in patients with proliferative diabetic retinopathy via the inhibition of IL-17 expression.
Accumulation of self-reactive naïve and memory B cell reveals sequential defects in B cell tolerance checkpoints in Sjögren's syndrome.
Quantitative reconstruction of leukocyte subsets using DNA methylation.
A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function.
Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 T cells.
Wang L, Yang X, Li D, Liang Z, Chen Y, Ma G, Wang Y, Li Y, Liang Y, Niu H
OncoTargets and therapy 2018;11:7229-7243
OncoTargets and therapy 2018;11:7229-7243
The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.
Ding JW, Zhou T, Zheng XX, Wang XA, Tong XH, Luo CY, Zhang ZQ, Yu B
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Acta Cardiologica Sinica 2018 Sep;34(5):399-408
Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D'Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
The Journal of clinical investigation 2017 Jan 3;127(1):169-182
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Protective effects of SIRT1 in patients with proliferative diabetic retinopathy via the inhibition of IL-17 expression.
Liu S, Lin YU, Liu X
Experimental and therapeutic medicine 2016 Jan;11(1):257-262
Experimental and therapeutic medicine 2016 Jan;11(1):257-262
Accumulation of self-reactive naïve and memory B cell reveals sequential defects in B cell tolerance checkpoints in Sjögren's syndrome.
Corsiero E, Sutcliffe N, Pitzalis C, Bombardieri M
PloS one 2014;9(12):e114575
PloS one 2014;9(12):e114575
Quantitative reconstruction of leukocyte subsets using DNA methylation.
Accomando WP, Wiencke JK, Houseman EA, Nelson HH, Kelsey KT
Genome biology 2014 Mar 5;15(3):R50
Genome biology 2014 Mar 5;15(3):R50
A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function.
Graves CL, Harden SW, LaPato M, Nelson M, Amador B, Sorenson H, Frazier CJ, Wallet SM
Journal of immunological methods 2014 Dec 1;414:20-31
Journal of immunological methods 2014 Dec 1;414:20-31
Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 T cells.
Wang H, Henry O, Distefano MD, Wang YC, Räikkönen J, Mönkkönen J, Tanaka Y, Morita CT
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1029-42
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1029-42
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with Mouse IgG2a kappa Isotype Control, FITC (Product # 11-4724-82) (blue histogram) or CD3 Monoclonal Antibody, FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye