12-0039-41

antibody from Invitrogen Antibodies
Targeting: CD247 CD3H, CD3Q, CD3Z
Provider product page for 12-0039-41

Antibody data

Product number
12-0039-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD3 Monoclonal Antibody (HIT3a), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
HIT3a
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD247 protein structure - 12-0039-41 shown in red.