12-2479-80

antibody from Invitrogen Antibodies
Targeting: CD247 CD3H, CD3Q, CD3Z
Provider product page for 12-2479-80

Antibody data

Product number
12-2479-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD247 (CD3 zeta) Monoclonal Antibody (6B10.2), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain. The CD3 complex is composed of three monomeric chains (gamma, delta, and ε) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the η subunit. Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, ε, and zeta chains comprise the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells. Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain. The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain. Therefore, flow cytometric analysis with this antibody requires intracellular staining. In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer. Applications Reported: This 6B10.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 6B10.2 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This atibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
6B10.2
Vial size
25 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
CD247 protein structure - 12-2479-80 shown in red.