Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-2479-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD247 (CD3 zeta) Monoclonal Antibody (6B10.2), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain. The CD3 complex is composed of three monomeric chains (gamma, delta, and ε) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the η subunit. Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, ε, and zeta chains comprise the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells. Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain. The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain. Therefore, flow cytometric analysis with this antibody requires intracellular staining. In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer. Applications Reported: This 6B10.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 6B10.2 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This atibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 6B10.2
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.
The prognostic value of the myeloid-mediated immunosuppression marker Arginase-1 in classic Hodgkin lymphoma.
Structural biology of the T-cell receptor: insights into receptor assembly, ligand recognition, and initiation of signaling.
T cell receptor zeta reconstitution fails to restore responses of T cells rendered hyporesponsive by tumor necrosis factor alpha.
Association of the human CD3-zeta chain with the alpha beta-T cell receptor/CD3 complex. Clues from a T cell variant with a mutated T cell receptor-alpha chain.
Fc gamma receptor type III (CD16) is included in the zeta NK receptor complex expressed by human natural killer cells.
The structure and signalling functions of the invariant T cell receptor components.
Cella M, Gamini R, Sécca C, Collins PL, Zhao S, Peng V, Robinette ML, Schettini J, Zaitsev K, Gordon W, Bando JK, Yomogida K, Cortez V, Fronick C, Fulton R, Lin LL, Gilfillan S, Flavell RA, Shan L, Artyomov MN, Bowman M, Oltz EM, Jelinsky SA, Colonna M
Nature immunology 2019 Aug;20(8):980-991
Nature immunology 2019 Aug;20(8):980-991
The prognostic value of the myeloid-mediated immunosuppression marker Arginase-1 in classic Hodgkin lymphoma.
Romano A, Parrinello NL, Vetro C, Tibullo D, Giallongo C, La Cava P, Chiarenza A, Motta G, Caruso AL, Villari L, Tripodo C, Cosentino S, Ippolito M, Consoli U, Gallamini A, Pileri S, Di Raimondo F
Oncotarget 2016 Oct 11;7(41):67333-67346
Oncotarget 2016 Oct 11;7(41):67333-67346
Structural biology of the T-cell receptor: insights into receptor assembly, ligand recognition, and initiation of signaling.
Wucherpfennig KW, Gagnon E, Call MJ, Huseby ES, Call ME
Cold Spring Harbor perspectives in biology 2010 Apr;2(4):a005140
Cold Spring Harbor perspectives in biology 2010 Apr;2(4):a005140
T cell receptor zeta reconstitution fails to restore responses of T cells rendered hyporesponsive by tumor necrosis factor alpha.
Clark JM, Annenkov AE, Panesar M, Isomäki P, Chernajovsky Y, Cope AP
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 10;101(6):1696-701
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 10;101(6):1696-701
Association of the human CD3-zeta chain with the alpha beta-T cell receptor/CD3 complex. Clues from a T cell variant with a mutated T cell receptor-alpha chain.
Geisler C, Schøller J, Wahi MA, Rubin B, Weiss A
Journal of immunology (Baltimore, Md. : 1950) 1990 Sep 15;145(6):1761-7
Journal of immunology (Baltimore, Md. : 1950) 1990 Sep 15;145(6):1761-7
Fc gamma receptor type III (CD16) is included in the zeta NK receptor complex expressed by human natural killer cells.
Anderson P, Caligiuri M, O'Brien C, Manley T, Ritz J, Schlossman SF
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2274-8
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2274-8
The structure and signalling functions of the invariant T cell receptor components.
Frank SJ, Samelson LE, Klausner RD
Seminars in immunology 1990 Mar;2(2):89-97
Seminars in immunology 1990 Mar;2(2):89-97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) followed by intracellular staining with 0.125 µg of Mouse IgG1 k Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Human CD247 (CD3 zeta) PE (right) using the Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye