Antibody data
- Antibody Data
- Antigen structure
- References [203]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [29]
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- Product number
- 16-0037-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
- Antibody clone number
- OKT3
- Concentration
- 1 mg/mL
Submitted references A reengineered common chain cytokine augments CD8+ T cell-dependent immunotherapy.
A purine metabolic checkpoint that prevents autoimmunity and autoinflammation.
Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor.
Cyclooxygenase-2 expressed hepatocellular carcinoma induces cytotoxic T lymphocytes exhaustion through M2 macrophage polarization.
Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma.
Pharmacokinetic Engineering of OX40-Blocking Anticalin Proteins Using Monomeric Plasma Half-Life Extension Domains.
Episomal reprogramming of Duchenne muscular dystrophy patients derived CD3(+) T cells towards induced pluripotent stem cells.
Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment.
Deciphering the state of immune silence in fatal COVID-19 patients.
A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade.
The Beneficial Role of Sunitinib in Tumor Immune Surveillance by Regulating Tumor PD-L1.
Reciprocal regulation of interleukin-17A and interleukin-22 secretion through aryl hydrocarbon receptor activation in CD4(+) T cells of patients with vitiligo.
Recurrent deletions in clonal hematopoiesis are driven by microhomology-mediated end joining.
Immune Monitoring during Therapy Reveals Activitory and Regulatory Immune Responses in High-Risk Neuroblastoma.
IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.
IL-12-Induced Immune Suppressive Deficit During CD8+ T-Cell Differentiation.
Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions.
IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.
Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1.
JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction.
Gene expression profile of CD14(+) blood monocytes following lifestyle-induced weight loss in individuals with metabolic syndrome.
Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.
IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis.
A Nonsense N -Terminus NFKB2 Mutation Leading to Haploinsufficiency in a Patient with a Predominantly Antibody Deficiency.
Unbiased Screens Show CD8(+) T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.
Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.
Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.
Prostaglandin E2 Reverses the Effects of DNA Methyltransferase Inhibitor and TGFB1 on the Conversion of Naive T Cells to iTregs.
T Cell Receptor Engineered Lymphocytes for Cancer Therapy.
Methodology for in vitro Assessment of Human T Cell Activation and Blockade.
High CD26 and Low CD94 Expression Identifies an IL-23 Responsive Vδ2(+) T Cell Subset with a MAIT Cell-like Transcriptional Profile.
Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer.
T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8(+) T cells in oral lichen planus.
A bispecific T cell engager targeting Glypican-1 redirects T cell cytolytic activity to kill prostate cancer cells.
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.
Reversal of pre-existing NGFR-driven tumor and immune therapy resistance.
CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression.
γδ T-cell Receptors Derived from Breast Cancer-Infiltrating T Lymphocytes Mediate Antitumor Reactivity.
Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligands.
HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane.
PD-1 Blockade Reinvigorates Bone Marrow CD8(+) T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.
Pomalidomide increases immune surface marker expression and immune recognition of oncovirus-infected cells.
Disruption of FOXP3-EZH2 Interaction Represents a Pathobiological Mechanism in Intestinal Inflammation.
Human cord blood (hCB)-CD34+ humanized mice fail to reject human acute myeloid leukemia cells.
Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.
Fetal T Cell Activation in the Amniotic Cavity during Preterm Labor: A Potential Mechanism for a Subset of Idiopathic Preterm Birth.
Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.
Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.
BRAF Targeting Sensitizes Resistant Melanoma to Cytotoxic T Cells.
HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers.
ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.
IL-17A increases MHC class I expression and promotes T cell activation in papillary thyroid cancer patients with coexistent Hashimoto's thyroiditis.
Triptolide reverses helper T cell inhibition and down-regulates IFN-γ induced PD-L1 expression in glioma cell lines.
Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung.
An increase in circulating B cells and B cell activation markers in peripheral blood is associated with cigarette smoking in a male cohort in Bangladesh.
Human T2R38 Bitter Taste Receptor Expression in Resting and Activated Lymphocytes.
Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Limited Cross-Linking of 4-1BB by 4-1BB Ligand and the Agonist Monoclonal Antibody Utomilumab.
Characterization and analysis of scFv-IgG bispecific antibody size variants.
Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection.
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Description of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver.
Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency.
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation.
Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.
A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association.
A library-based screening method identifies neoantigen-reactive T cells in peripheral blood prior to relapse of ovarian cancer.
T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
A Multikinase and DNA-PK Inhibitor Combination Immunomodulates Melanomas, Suppresses Tumor Progression, and Enhances Immunotherapies.
Screening of an FDA-approved compound library identifies levosimendan as a novel anti-HIV-1 agent that inhibits viral transcription.
RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization.
Micropipette force probe to quantify single-cell force generation: application to T-cell activation.
Pooled CRISPR screening with single-cell transcriptome readout.
Serum Gp96 is a chaperone of complement-C3 during graft-versus-host disease.
Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity.
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.
Predominance of weakly cytotoxic, T-bet(Low)Eomes(Neg) CD8(+) T-cells in human gastrointestinal mucosa: implications for HIV infection.
Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.
Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.
Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity.
Discovery and characterization of COVA322, a clinical-stage bispecific TNF/IL-17A inhibitor for the treatment of inflammatory diseases.
Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells.
CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Enhanced recruitment of genetically modified CX3CR1-positive human T cells into Fractalkine/CX3CL1 expressing tumors: importance of the chemokine gradient.
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy.
Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types.
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.
Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data.
T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.
LFA-1 Engagement Triggers T Cell Polarization at the HIV-1 Virological Synapse.
Factor H uptake regulates intracellular C3 activation during apoptosis and decreases the inflammatory potential of nucleosomes.
Obesity decreases B cell responses in young and elderly individuals.
Cytokine-induced killer cell therapy for the treatment of primary hepatocellular carcinoma subsequent to liver transplantation: A case report.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.
Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes.
Effect of cytokine-induced killer cells on immune function in patients with lung cancer.
Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab.
RAX2: a genome-wide detection method of condition-associated transcription variation.
IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1.
Soluble PD-1 aggravates progression of collagen-induced arthritis through Th1 and Th17 pathways.
HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes.
Noninvasive detection of tumor-infiltrating T cells by PET reporter imaging.
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Wide-scale quantitative phosphoproteomic analysis reveals that cold treatment of T cells closely mimics soluble antibody stimulation.
Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.
Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints.
SRC homology 2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76) N-terminal tyrosine residues regulate a dynamic signaling equilibrium involving feedback of proximal T-cell receptor (TCR) signaling.
Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
A novel human STAT3 mutation presents with autoimmunity involving Th17 hyperactivation.
Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP.
Collagen induces maturation of human monocyte-derived dendritic cells by signaling through osteoclast-associated receptor.
Analysis of ancestral and functionally relevant CD5 variants in systemic lupus erythematosus patients.
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Cell-based measurements of mitochondrial function in human subjects.
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Immunosuppressive activity of an aqueous Viola tricolor herbal extract.
Apremilast is a selective PDE4 inhibitor with regulatory effects on innate immunity.
Synergistic effects of IL-4 and TNFα on the induction of B7-H1 in renal cell carcinoma cells inhibiting allogeneic T cell proliferation.
Pan-Bcl-2 inhibitor, GX15-070 (obatoclax), decreases human T regulatory lymphocytes while preserving effector T lymphocytes: a rationale for its use in combination immunotherapy.
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Prostaglandin E2 promotes survival of naive UCB T cells via the Wnt/β-catenin pathway and alters immune reconstitution after UCBT.
Long-lasting disease stabilization in the absence of toxicity in metastatic lung cancer patients vaccinated with an epitope derived from indoleamine 2,3 dioxygenase.
A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.
Alterations in both the activatory and inhibitory potential of peripheral blood CD4+ T cells in rheumatoid arthritis patients correlate with disease progression.
Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.
IL-10 conditioning of human skin affects the distribution of migratory dendritic cell subsets and functional T cell differentiation.
Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.
GFP affects human T cell activation and cytokine production following in vitro stimulation.
An efficient low cost method for gene transfer to T lymphocytes.
Production and first-in-man use of T cells engineered to express a HSVTK-CD34 sort-suicide gene.
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
NF-κB is activated in CD4+ iNKT cells by sickle cell disease and mediates rapid induction of adenosine A2A receptors.
Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Functional characterization of EZH2β reveals the increased complexity of EZH2 isoforms involved in the regulation of mammalian gene expression.
Thrombospondin-1 is a CD47-dependent endogenous inhibitor of hydrogen sulfide signaling in T cell activation.
The infectious synapse formed between mature dendritic cells and CD4(+) T cells is independent of the presence of the HIV-1 envelope glycoprotein.
Functional characterization of a STAT3-dependent dendritic cell-derived CD14(+) cell population arising upon IL-10-driven maturation.
Ectonucleotidase CD38 demarcates regulatory, memory-like CD8+ T cells with IFN-γ-mediated suppressor activities.
Stimulation of Wnt/ß-catenin pathway in human CD8+ T lymphocytes from blood and lung tumors leads to a shared young/memory phenotype.
Recombinant expression of margatoxin and agitoxin-2 in Pichia pastoris: an efficient method for production of KV1.3 channel blockers.
MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway.
Decrease in proportion of CD19+ CD24(hi) CD27+ B cells and impairment of their suppressive function in Graves' disease.
Quantitative phosphoproteomics reveals SLP-76 dependent regulation of PAG and Src family kinases in T cells.
Granulocyte macrophage colony-stimulating factor treatment of a patient in myasthenic crisis: effects on regulatory T cells.
An NKp30-based chimeric antigen receptor promotes T cell effector functions and antitumor efficacy in vivo.
Trauma patients' elevated tumor necrosis related apoptosis inducing ligand (TRAIL) contributes to increased T cell apoptosis.
Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system.
A C-terminally amidated analogue of ShK is a potent and selective blocker of the voltage-gated potassium channel Kv1.3.
IL-7 drives Th1 and Th17 cytokine production in patients with primary SS despite an increase in CD4 T cells lacking the IL-7Rα.
Myeloid suppressor cells induced by hepatitis C virus suppress T-cell responses through the production of reactive oxygen species.
Hydrogen sulfide is an endogenous potentiator of T cell activation.
Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease.
Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Tracking immune cell proliferation and cytotoxic potential using flow cytometry.
Involvement of CCR6/CCL20/IL-17 axis in NSCLC disease progression.
Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
Choice of resident costimulatory molecule can influence cell fate in human naïve CD4+ T cell differentiation.
Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis.
Anti-leukemia effect of ex vivo expanded DNT cells from AML patients: a potential novel autologous T-cell adoptive immunotherapy.
The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor.
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Next-generation insights into regulatory T cells: expression profiling and FoxP3 occupancy in Human.
B7-h4 expression in human melanoma: its association with patients' survival and antitumor immune response.
Reduced thymic output, cell cycle abnormalities, and increased apoptosis of T lymphocytes in patients with cartilage-hair hypoplasia.
Positive T cell co-stimulation by TLR7/8 ligands is dependent on the cellular environment.
Hypoxia potentiates glioma-mediated immunosuppression.
The vitamin D analog, TX527, promotes a human CD4+CD25highCD127low regulatory T cell profile and induces a migratory signature specific for homing to sites of inflammation.
Interleukin-21 and cellular activation concurrently induce potent cytotoxic function and promote antiviral activity in human CD8 T cells.
High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses.
Dichotomy between factors inducing the immunosuppressive enzyme IL-4-induced gene 1 (IL4I1) in B lymphocytes and mononuclear phagocytes.
The role of Vδ2-negative γδ T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation.
Impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets.
Glioma cancer stem cells induce immunosuppressive macrophages/microglia.
T-cell receptor early signalling complex activation in response to interferon-alpha receptor stimulation.
MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1.
Glioblastoma cancer-initiating cells inhibit T-cell proliferation and effector responses by the signal transducers and activators of transcription 3 pathway.
The oncogenic BRAF kinase inhibitor PLX4032/RG7204 does not affect the viability or function of human lymphocytes across a wide range of concentrations.
miR-155 inhibition sensitizes CD4+ Th cells for TREG mediated suppression.
A new approach for quantitative phosphoproteomic dissection of signaling pathways applied to T cell receptor activation.
Highly active microbial phosphoantigen induces rapid yet sustained MEK/Erk- and PI-3K/Akt-mediated signal transduction in anti-tumor human gammadelta T-cells.
Auf1/Hnrnpd-deficient mice develop pruritic inflammatory skin disease.
Absence of leucine zipper in the natural FOXP3Delta2Delta7 isoform does not affect dimerization but abrogates suppressive capacity.
Clinical heterogeneity can hamper the diagnosis of patients with ZAP70 deficiency.
Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.
Human activated T lymphocytes modulate IDO expression in tumors through Th1/Th2 balance.
Identification of heme oxygenase-1-specific regulatory CD8+ T cells in cancer patients.
The induction of APC with a distinct tolerogenic phenotype via contact-dependent STAT3 activation.
Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases.
The effect of artificial antigen-presenting cells with preclustered anti-CD28/-CD3/-LFA-1 monoclonal antibodies on the induction of ex vivo expansion of functional human antitumor T cells.
Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-kappaB activation.
Structure, inhibitor, and regulatory mechanism of Lyp, a lymphoid-specific tyrosine phosphatase implicated in autoimmune diseases.
Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands.
Banerjee A, Li D, Guo Y, Mei Z, Lau C, Chen K, Westwick J, Klauda JB, Schrum A, Lazear ER, Krupnick AS
JCI insight 2022 May 23;7(10)
JCI insight 2022 May 23;7(10)
A purine metabolic checkpoint that prevents autoimmunity and autoinflammation.
Saveljeva S, Sewell GW, Ramshorn K, Cader MZ, West JA, Clare S, Haag LM, de Almeida Rodrigues RP, Unger LW, Iglesias-Romero AB, Holland LM, Bourges C, Md-Ibrahim MN, Jones JO, Blumberg RS, Lee JC, Kaneider NC, Lawley TD, Bradley A, Dougan G, Kaser A
Cell metabolism 2022 Jan 4;34(1):106-124.e10
Cell metabolism 2022 Jan 4;34(1):106-124.e10
Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor.
Rode AKO, Buus TB, Mraz V, Al-Jaberi FAH, Lopez DV, Ford SL, Hennen S, Eliasen IP, Klewe IV, Gharehdaghi L, Dragan A, Rosenkilde MM, Woetmann A, Skov L, Ødum N, Bonefeld CM, Kongsbak-Wismann M, Geisler C
Cells 2022 Aug 19;11(16)
Cells 2022 Aug 19;11(16)
Cyclooxygenase-2 expressed hepatocellular carcinoma induces cytotoxic T lymphocytes exhaustion through M2 macrophage polarization.
Xun X, Zhang C, Wang S, Hu S, Xiang X, Cheng Q, Li Z, Wang Y, Zhu J
American journal of translational research 2021;13(5):4360-4375
American journal of translational research 2021;13(5):4360-4375
Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma.
Wang T, Zhou Q, Shang Y
Journal of innate immunity 2021;13(1):38-48
Journal of innate immunity 2021;13(1):38-48
Pharmacokinetic Engineering of OX40-Blocking Anticalin Proteins Using Monomeric Plasma Half-Life Extension Domains.
Siegemund M, Oak P, Hansbauer EM, Allersdorfer A, Utschick K, Winter A, Grasmüller C, Galler G, Mayer JP, Weiche B, Prassler J, Kontermann RE, Rothe C
Frontiers in pharmacology 2021;12:759337
Frontiers in pharmacology 2021;12:759337
Episomal reprogramming of Duchenne muscular dystrophy patients derived CD3(+) T cells towards induced pluripotent stem cells.
Zehravi M, Wahid M, Ashraf J
Pakistan journal of medical sciences 2021 Mar-Apr;37(2):432-438
Pakistan journal of medical sciences 2021 Mar-Apr;37(2):432-438
Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment.
Rubio-Perez C, Planas-Rigol E, Trincado JL, Bonfill-Teixidor E, Arias A, Marchese D, Moutinho C, Serna G, Pedrosa L, Iurlaro R, Martínez-Ricarte F, Escudero L, Cordero E, Cicuendez M, Ruiz S, Parra G, Nuciforo P, Gonzalez J, Pineda E, Sahuquillo J, Tabernero J, Heyn H, Seoane J
Nature communications 2021 Mar 8;12(1):1503
Nature communications 2021 Mar 8;12(1):1503
Deciphering the state of immune silence in fatal COVID-19 patients.
Bost P, De Sanctis F, Canè S, Ugel S, Donadello K, Castellucci M, Eyal D, Fiore A, Anselmi C, Barouni RM, Trovato R, Caligola S, Lamolinara A, Iezzi M, Facciotti F, Mazzariol A, Gibellini D, De Nardo P, Tacconelli E, Gottin L, Polati E, Schwikowski B, Amit I, Bronte V
Nature communications 2021 Mar 5;12(1):1428
Nature communications 2021 Mar 5;12(1):1428
A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade.
Geuijen C, Tacken P, Wang LC, Klooster R, van Loo PF, Zhou J, Mondal A, Liu YB, Kramer A, Condamine T, Volgina A, Hendriks LJA, van der Maaden H, Rovers E, Engels S, Fransen F, den Blanken-Smit R, Zondag-van der Zande V, Basmeleh A, Bartelink W, Kulkarni A, Marissen W, Huang CY, Hall L, Harvey S, Kim S, Martinez M, O'Brien S, Moon E, Albelda S, Kanellopoulou C, Stewart S, Nastri H, Bakker ABH, Scherle P, Logtenberg T, Hollis G, de Kruif J, Huber R, Mayes PA, Throsby M
Nature communications 2021 Jul 21;12(1):4445
Nature communications 2021 Jul 21;12(1):4445
The Beneficial Role of Sunitinib in Tumor Immune Surveillance by Regulating Tumor PD-L1.
Li H, Kuang X, Liang L, Ye Y, Zhang Y, Li J, Ma F, Tao J, Lei G, Zhao S, Su J, Yang N, Peng C, Xu X, Hung MC, Han L, Liu H, Liu J, Chen X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Jan;8(2):2001596
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Jan;8(2):2001596
Reciprocal regulation of interleukin-17A and interleukin-22 secretion through aryl hydrocarbon receptor activation in CD4(+) T cells of patients with vitiligo.
Liu B, Xie Y, Mei X, Sun Y, Shi W, Wu Z
Experimental and therapeutic medicine 2021 Feb;21(2):158
Experimental and therapeutic medicine 2021 Feb;21(2):158
Recurrent deletions in clonal hematopoiesis are driven by microhomology-mediated end joining.
Feldman T, Bercovich A, Moskovitz Y, Chapal-Ilani N, Mitchell A, Medeiros JJF, Biezuner T, Kaushansky N, Minden MD, Gupta V, Milyavsky M, Livneh Z, Tanay A, Shlush LI
Nature communications 2021 Apr 28;12(1):2455
Nature communications 2021 Apr 28;12(1):2455
Immune Monitoring during Therapy Reveals Activitory and Regulatory Immune Responses in High-Risk Neuroblastoma.
Szanto CL, Cornel AM, Tamminga SM, Delemarre EM, de Koning CCH, van den Beemt DAMH, Dunnebach E, Tas ML, Dierselhuis MP, Tytgat LGAM, van Noesel MM, Kraal KCJM, Boelens JJ, Huitema ADR, Nierkens S
Cancers 2021 Apr 26;13(9)
Cancers 2021 Apr 26;13(9)
IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.
Hu JQ, Lei BW, Wen D, Ma B, Zhang TT, Lu ZW, Wei WJ, Wang YL, Wang Y, Li DS, Ji QH, Liao T
Journal of Cancer 2020;11(14):4250-4260
Journal of Cancer 2020;11(14):4250-4260
IL-12-Induced Immune Suppressive Deficit During CD8+ T-Cell Differentiation.
Renavikar PS, Sinha S, Brate AA, Borcherding N, Crawford MP, Steward-Tharp SM, Karandikar NJ
Frontiers in immunology 2020;11:568630
Frontiers in immunology 2020;11:568630
Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions.
Ferrara M, Chialli G, Ferreira LM, Ruggieri E, Careccia G, Preti A, Piccirillo R, Bianchi ME, Sitia G, Venereau E
Frontiers in immunology 2020;11:1122
Frontiers in immunology 2020;11:1122
IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.
Sadik A, Somarribas Patterson LF, Öztürk S, Mohapatra SR, Panitz V, Secker PF, Pfänder P, Loth S, Salem H, Prentzell MT, Berdel B, Iskar M, Faessler E, Reuter F, Kirst I, Kalter V, Foerster KI, Jäger E, Guevara CR, Sobeh M, Hielscher T, Poschet G, Reinhardt A, Hassel JC, Zapatka M, Hahn U, von Deimling A, Hopf C, Schlichting R, Escher BI, Burhenne J, Haefeli WE, Ishaque N, Böhme A, Schäuble S, Thedieck K, Trump S, Seiffert M, Opitz CA
Cell 2020 Sep 3;182(5):1252-1270.e34
Cell 2020 Sep 3;182(5):1252-1270.e34
Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1.
Ye Y, Kuang X, Xie Z, Liang L, Zhang Z, Zhang Y, Ma F, Gao Q, Chang R, Lee HH, Zhao S, Su J, Li H, Peng J, Chen H, Yin M, Peng C, Yang N, Wang J, Liu J, Liu H, Han L, Chen X
Genome medicine 2020 Sep 28;12(1):83
Genome medicine 2020 Sep 28;12(1):83
JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction.
Simula L, Corrado M, Accordi B, Di Rita A, Nazio F, Antonucci Y, Di Daniele A, Caicci F, Caruana I, Soriano ME, Pigazzi M, Locatelli F, Cecconi F, Campello S
Cell death and differentiation 2020 Oct;27(10):2749-2767
Cell death and differentiation 2020 Oct;27(10):2749-2767
Gene expression profile of CD14(+) blood monocytes following lifestyle-induced weight loss in individuals with metabolic syndrome.
Biemann R, Roomp K, Noor F, Krishnan S, Li Z, Shahzad K, Borucki K, Luley C, Schneider JG, Isermann B
Scientific reports 2020 Oct 20;10(1):17855
Scientific reports 2020 Oct 20;10(1):17855
Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.
Lee J, Sheen JH, Lim O, Lee Y, Ryu J, Shin D, Kim YY, Kim M
Scientific reports 2020 Oct 20;10(1):17753
Scientific reports 2020 Oct 20;10(1):17753
IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis.
Lu ZW, Hu JQ, Liu WL, Wen D, Wei WJ, Wang YL, Wang Y, Liao T, Ji QH
Endocrinology 2020 Oct 1;161(10)
Endocrinology 2020 Oct 1;161(10)
A Nonsense N -Terminus NFKB2 Mutation Leading to Haploinsufficiency in a Patient with a Predominantly Antibody Deficiency.
Kuehn HS, Bernasconi A, Niemela JE, Almejun MB, Gallego WAF, Goel S, Stoddard JL, Sánchez RGP, Franco CAA, Oleastro M, Grunebaum E, Ballas Z, Cunningham-Rundles C, Fleisher TA, Franco JL, Danielian S, Rosenzweig SD
Journal of clinical immunology 2020 Nov;40(8):1093-1101
Journal of clinical immunology 2020 Nov;40(8):1093-1101
Unbiased Screens Show CD8(+) T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.
Ferretti AP, Kula T, Wang Y, Nguyen DMV, Weinheimer A, Dunlap GS, Xu Q, Nabilsi N, Perullo CR, Cristofaro AW, Whitton HJ, Virbasius A, Olivier KJ Jr, Buckner LR, Alistar AT, Whitman ED, Bertino SA, Chattopadhyay S, MacBeath G
Immunity 2020 Nov 17;53(5):1095-1107.e3
Immunity 2020 Nov 17;53(5):1095-1107.e3
Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.
Ma Y, Yang Q, Fan M, Zhang L, Gu Y, Jia W, Li Z, Wang F, Li YX, Wang J, Li R, Shao X, Wang YL
Cell proliferation 2020 May;53(5):e12802
Cell proliferation 2020 May;53(5):e12802
Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.
Ko JH, Kim HJ, Jeong HJ, Lee HJ, Oh JY
Cell reports 2020 Mar 17;30(11):3806-3820.e6
Cell reports 2020 Mar 17;30(11):3806-3820.e6
Prostaglandin E2 Reverses the Effects of DNA Methyltransferase Inhibitor and TGFB1 on the Conversion of Naive T Cells to iTregs.
Sahin M, Sahin E
Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 2020 Jun;47(3):244-253
Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 2020 Jun;47(3):244-253
T Cell Receptor Engineered Lymphocytes for Cancer Therapy.
Rollins MR, Spartz EJ, Stromnes IM
Current protocols in immunology 2020 Jun;129(1):e97
Current protocols in immunology 2020 Jun;129(1):e97
Methodology for in vitro Assessment of Human T Cell Activation and Blockade.
Podojil JR, Ifergan I, Chiang MY, Meeks JJ, Miller SD
Bio-protocol 2020 Jun 5;10(11):e3644
Bio-protocol 2020 Jun 5;10(11):e3644
High CD26 and Low CD94 Expression Identifies an IL-23 Responsive Vδ2(+) T Cell Subset with a MAIT Cell-like Transcriptional Profile.
Wragg KM, Tan HX, Kristensen AB, Nguyen-Robertson CV, Kelleher AD, Parsons MS, Wheatley AK, Berzins SP, Pellicci DG, Kent SJ, Juno JA
Cell reports 2020 Jun 16;31(11):107773
Cell reports 2020 Jun 16;31(11):107773
Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer.
Salazar Y, Zheng X, Brunn D, Raifer H, Picard F, Zhang Y, Winter H, Guenther S, Weigert A, Weigmann B, Dumoutier L, Renauld JC, Waisman A, Schmall A, Tufman A, Fink L, Brüne B, Bopp T, Grimminger F, Seeger W, Pullamsetti SS, Huber M, Savai R
The Journal of clinical investigation 2020 Jul 1;130(7):3560-3575
The Journal of clinical investigation 2020 Jul 1;130(7):3560-3575
T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8(+) T cells in oral lichen planus.
Yang JY, Zhang J, Lu R, Tan YQ, Du GF, Zhou G
Journal of cellular and molecular medicine 2020 Dec;24(23):14086-14098
Journal of cellular and molecular medicine 2020 Dec;24(23):14086-14098
A bispecific T cell engager targeting Glypican-1 redirects T cell cytolytic activity to kill prostate cancer cells.
Lund ME, Howard CB, Thurecht KJ, Campbell DH, Mahler SM, Walsh BJ
BMC cancer 2020 Dec 10;20(1):1214
BMC cancer 2020 Dec 10;20(1):1214
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.
Su Y, Chen D, Yuan D, Lausted C, Choi J, Dai CL, Voillet V, Duvvuri VR, Scherler K, Troisch P, Baloni P, Qin G, Smith B, Kornilov SA, Rostomily C, Xu A, Li J, Dong S, Rothchild A, Zhou J, Murray K, Edmark R, Hong S, Heath JE, Earls J, Zhang R, Xie J, Li S, Roper R, Jones L, Zhou Y, Rowen L, Liu R, Mackay S, O'Mahony DS, Dale CR, Wallick JA, Algren HA, Zager MA, ISB-Swedish COVID19 Biobanking Unit, Wei W, Price ND, Huang S, Subramanian N, Wang K, Magis AT, Hadlock JJ, Hood L, Aderem A, Bluestone JA, Lanier LL, Greenberg PD, Gottardo R, Davis MM, Goldman JD, Heath JR
Cell 2020 Dec 10;183(6):1479-1495.e20
Cell 2020 Dec 10;183(6):1479-1495.e20
Reversal of pre-existing NGFR-driven tumor and immune therapy resistance.
Boshuizen J, Vredevoogd DW, Krijgsman O, Ligtenberg MA, Blankenstein S, de Bruijn B, Frederick DT, Kenski JCN, Parren M, Brüggemann M, Madu MF, Rozeman EA, Song JY, Horlings HM, Blank CU, van Akkooi ACJ, Flaherty KT, Boland GM, Peeper DS
Nature communications 2020 Aug 7;11(1):3946
Nature communications 2020 Aug 7;11(1):3946
CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression.
Crawford MP, Sinha S, Renavikar PS, Borcherding N, Karandikar NJ
Proceedings of the National Academy of Sciences of the United States of America 2020 Aug 11;117(32):19408-19414
Proceedings of the National Academy of Sciences of the United States of America 2020 Aug 11;117(32):19408-19414
γδ T-cell Receptors Derived from Breast Cancer-Infiltrating T Lymphocytes Mediate Antitumor Reactivity.
Janssen A, Villacorta Hidalgo J, Beringer DX, van Dooremalen S, Fernando F, van Diest E, Terrizi AR, Bronsert P, Kock S, Schmitt-Gräff A, Werner M, Heise K, Follo M, Straetemans T, Sebestyen Z, Chudakov DM, Kasatskaya SA, Frenkel FE, Ravens S, Spierings E, Prinz I, Küppers R, Malkovsky M, Fisch P, Kuball J
Cancer immunology research 2020 Apr;8(4):530-543
Cancer immunology research 2020 Apr;8(4):530-543
Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligands.
Chang ZL, Hou AJ, Chen YY
Nature protocols 2020 Apr;15(4):1507-1524
Nature protocols 2020 Apr;15(4):1507-1524
HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane.
Li WF, Aryal M, Shu ST, Smithgall TE
The Journal of biological chemistry 2020 Apr 10;295(15):5163-5174
The Journal of biological chemistry 2020 Apr 10;295(15):5163-5174
PD-1 Blockade Reinvigorates Bone Marrow CD8(+) T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.
Kwon M, Kim CG, Lee H, Cho H, Kim Y, Lee EC, Choi SJ, Park J, Seo IH, Bogen B, Song IC, Jo DY, Kim JS, Park SH, Choi I, Choi YS, Shin EC
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1644-1655
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1644-1655
Pomalidomide increases immune surface marker expression and immune recognition of oncovirus-infected cells.
Davis DA, Shrestha P, Aisabor AI, Stream A, Galli V, Pise-Masison CA, Tagawa T, Ziegelbauer JM, Franchini G, Yarchoan R
Oncoimmunology 2019;8(2):e1546544
Oncoimmunology 2019;8(2):e1546544
Disruption of FOXP3-EZH2 Interaction Represents a Pathobiological Mechanism in Intestinal Inflammation.
Bamidele AO, Svingen PA, Sagstetter MR, Sarmento OF, Gonzalez M, Braga Neto MB, Kugathasan S, Lomberk G, Urrutia RA, Faubion WA Jr
Cellular and molecular gastroenterology and hepatology 2019;7(1):55-71
Cellular and molecular gastroenterology and hepatology 2019;7(1):55-71
Human cord blood (hCB)-CD34+ humanized mice fail to reject human acute myeloid leukemia cells.
Tanaskovic O, Verga Falzacappa MV, Pelicci PG
PloS one 2019;14(9):e0217345
PloS one 2019;14(9):e0217345
Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.
Esfahani K, Al-Aubodah TA, Thebault P, Lapointe R, Hudson M, Johnson NA, Baran D, Bhulaiga N, Takano T, Cailhier JF, Piccirillo CA, Miller WH
Nature communications 2019 Oct 17;10(1):4712
Nature communications 2019 Oct 17;10(1):4712
Fetal T Cell Activation in the Amniotic Cavity during Preterm Labor: A Potential Mechanism for a Subset of Idiopathic Preterm Birth.
Gomez-Lopez N, Romero R, Xu Y, Miller D, Arenas-Hernandez M, Garcia-Flores V, Panaitescu B, Galaz J, Hsu CD, Para R, Berry SM
Journal of immunology (Baltimore, Md. : 1950) 2019 Oct 1;203(7):1793-1807
Journal of immunology (Baltimore, Md. : 1950) 2019 Oct 1;203(7):1793-1807
Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.
Rossatti P, Ziegler L, Schregle R, Betzler VM, Ecker M, Rossy J
Cells 2019 Nov 4;8(11)
Cells 2019 Nov 4;8(11)
Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.
Levring TB, Kongsbak-Wismann M, Rode AKO, Al-Jaberi FAH, Lopez DV, Met Ö, Woetmann A, Bonefeld CM, Ødum N, Geisler C
Scientific reports 2019 Nov 13;9(1):16725
Scientific reports 2019 Nov 13;9(1):16725
BRAF Targeting Sensitizes Resistant Melanoma to Cytotoxic T Cells.
Atay C, Kwak T, Lavilla-Alonso S, Donthireddy L, Richards A, Moberg V, Pilon-Thomas S, Schell M, Messina JL, Rebecca VW, Xiao M, Tan J, Zhang G, Weber JS, Herlyn M, Sarnaik AA, Gabrilovich DI
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 May 1;25(9):2783-2794
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 May 1;25(9):2783-2794
HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers.
Mo X, Tang C, Niu Q, Ma T, Du Y, Fu H
Cell chemical biology 2019 Mar 21;26(3):331-339.e3
Cell chemical biology 2019 Mar 21;26(3):331-339.e3
ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.
Cao Y, Trillo-Tinoco J, Sierra RA, Anadon C, Dai W, Mohamed E, Cen L, Costich TL, Magliocco A, Marchion D, Klar R, Michel S, Jaschinski F, Reich RR, Mehrotra S, Cubillos-Ruiz JR, Munn DH, Conejo-Garcia JR, Rodriguez PC
Nature communications 2019 Mar 20;10(1):1280
Nature communications 2019 Mar 20;10(1):1280
IL-17A increases MHC class I expression and promotes T cell activation in papillary thyroid cancer patients with coexistent Hashimoto's thyroiditis.
Han LT, Hu JQ, Ma B, Wen D, Zhang TT, Lu ZW, Wei WJ, Wang YL, Wang Y, Liao T, Ji QH
Diagnostic pathology 2019 Jun 4;14(1):52
Diagnostic pathology 2019 Jun 4;14(1):52
Triptolide reverses helper T cell inhibition and down-regulates IFN-γ induced PD-L1 expression in glioma cell lines.
Zhang L, Yu JS
Journal of neuro-oncology 2019 Jul;143(3):429-436
Journal of neuro-oncology 2019 Jul;143(3):429-436
Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung.
Wang W, Cohen JA, Wallrapp A, Trieu KG, Barrios J, Shao F, Krishnamoorthy N, Kuchroo VK, Jones MR, Fine A, Bai Y, Ai X
Immunity 2019 Dec 17;51(6):1102-1118.e7
Immunity 2019 Dec 17;51(6):1102-1118.e7
An increase in circulating B cells and B cell activation markers in peripheral blood is associated with cigarette smoking in a male cohort in Bangladesh.
Burchiel SW, Lauer FT, Factor-Litvak P, Liu X, Santella RM, Islam T, Eunus M, Alam N, Islam T, Rahman M, Ahmed A, Ahsan H, Graziano J, Parvez F
Toxicology and applied pharmacology 2019 Dec 1;384:114783
Toxicology and applied pharmacology 2019 Dec 1;384:114783
Human T2R38 Bitter Taste Receptor Expression in Resting and Activated Lymphocytes.
Tran HTT, Herz C, Ruf P, Stetter R, Lamy E
Frontiers in immunology 2018;9:2949
Frontiers in immunology 2018;9:2949
Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.
Pan H, Liu J, Deng W, Xing J, Li Q, Wang Z
International journal of nanomedicine 2018;13:3189-3201
International journal of nanomedicine 2018;13:3189-3201
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Idelson M, Alper R, Obolensky A, Yachimovich-Cohen N, Rachmilewitz J, Ejzenberg A, Beider E, Banin E, Reubinoff B
Stem cell reports 2018 Sep 11;11(3):681-695
Stem cell reports 2018 Sep 11;11(3):681-695
Limited Cross-Linking of 4-1BB by 4-1BB Ligand and the Agonist Monoclonal Antibody Utomilumab.
Li Y, Tan S, Zhang C, Chai Y, He M, Zhang CW, Wang Q, Tong Z, Liu K, Lei Y, Liu WJ, Liu Y, Tian Z, Cao X, Yan J, Qi J, Tien P, Gao S, Gao GF
Cell reports 2018 Oct 23;25(4):909-920.e4
Cell reports 2018 Oct 23;25(4):909-920.e4
Characterization and analysis of scFv-IgG bispecific antibody size variants.
Cao M, Wang C, Chung WK, Motabar D, Wang J, Christian E, Lin S, Hunter A, Wang X, Liu D
mAbs 2018 Nov-Dec;10(8):1236-1247
mAbs 2018 Nov-Dec;10(8):1236-1247
Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection.
Song H, Giorgi EE, Ganusov VV, Cai F, Athreya G, Yoon H, Carja O, Hora B, Hraber P, Romero-Severson E, Jiang C, Li X, Wang S, Li H, Salazar-Gonzalez JF, Salazar MG, Goonetilleke N, Keele BF, Montefiori DC, Cohen MS, Shaw GM, Hahn BH, McMichael AJ, Haynes BF, Korber B, Bhattacharya T, Gao F
Nature communications 2018 May 15;9(1):1928
Nature communications 2018 May 15;9(1):1928
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Seki A, Rutz S
The Journal of experimental medicine 2018 Mar 5;215(3):985-997
The Journal of experimental medicine 2018 Mar 5;215(3):985-997
Description of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver.
Shi Y, Zhang P, Wang G, Liu X, Sun X, Zhang X, Li H, Qi J, Ding L, Li T, Zhang R, Chen Y, Zhou J, Lv G, Tu Z
Oncotarget 2018 Mar 20;9(21):15552-15565
Oncotarget 2018 Mar 20;9(21):15552-15565
Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency.
Boutboul D, Kuehn HS, Van de Wyngaert Z, Niemela JE, Callebaut I, Stoddard J, Lenoir C, Barlogis V, Farnarier C, Vely F, Yoshida N, Kojima S, Kanegane H, Hoshino A, Hauck F, Lhermitte L, Asnafi V, Roehrs P, Chen S, Verbsky JW, Calvo KR, Husami A, Zhang K, Roberts J, Amrol D, Sleaseman J, Hsu AP, Holland SM, Marsh R, Fischer A, Fleisher TA, Picard C, Latour S, Rosenzweig SD
The Journal of clinical investigation 2018 Jul 2;128(7):3071-3087
The Journal of clinical investigation 2018 Jul 2;128(7):3071-3087
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation.
Compeer EB, Kraus F, Ecker M, Redpath G, Amiezer M, Rother N, Nicovich PR, Kapoor-Kaushik N, Deng Q, Samson GPB, Yang Z, Lou J, Carnell M, Vartoukian H, Gaus K, Rossy J
Nature communications 2018 Apr 23;9(1):1597
Nature communications 2018 Apr 23;9(1):1597
Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.
Carpier JM, Zucchetti AE, Bataille L, Dogniaux S, Shafaq-Zadah M, Bardin S, Lucchino M, Maurin M, Joannas LD, Magalhaes JG, Johannes L, Galli T, Goud B, Hivroz C
The Journal of experimental medicine 2018 Apr 2;215(4):1245-1265
The Journal of experimental medicine 2018 Apr 2;215(4):1245-1265
A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association.
Huang H, Liu S, Jean M, Simpson S, Huang H, Merkley M, Hayashi T, Kong W, Rodríguez-Sánchez I, Zhang X, Yosief HO, Miao H, Que J, Kobie JJ, Bradner J, Santoso NG, Zhang W, Zhu J
Frontiers in microbiology 2017;8:1035
Frontiers in microbiology 2017;8:1035
A library-based screening method identifies neoantigen-reactive T cells in peripheral blood prior to relapse of ovarian cancer.
Martin SD, Wick DA, Nielsen JS, Little N, Holt RA, Nelson BH
Oncoimmunology 2017;7(1):e1371895
Oncoimmunology 2017;7(1):e1371895
T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
Bughani U, Saha A, Kuriakose A, Nair R, Sadashivarao RB, Venkataraman R, Patel S, Deshchougule AT, S SK, Montero E, Pai HV, Palanivelu DV, Melarkode R, Nair P
PloS one 2017;12(7):e0180088
PloS one 2017;12(7):e0180088
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
Hernández Del Pino RE, Pellegrini JM, Rovetta AI, Peña D, Álvarez GI, Rolandelli A, Musella RM, Palmero DJ, Malbran A, Pasquinelli V, García VE
Immunology and cell biology 2017 Sep;95(8):716-728
Immunology and cell biology 2017 Sep;95(8):716-728
A Multikinase and DNA-PK Inhibitor Combination Immunomodulates Melanomas, Suppresses Tumor Progression, and Enhances Immunotherapies.
Tsai AK, Khan AY, Worgo CE, Wang LL, Liang Y, Davila E
Cancer immunology research 2017 Sep;5(9):790-803
Cancer immunology research 2017 Sep;5(9):790-803
Screening of an FDA-approved compound library identifies levosimendan as a novel anti-HIV-1 agent that inhibits viral transcription.
Hayashi T, Jean M, Huang H, Simpson S, Santoso NG, Zhu J
Antiviral research 2017 Oct;146:76-85
Antiviral research 2017 Oct;146:76-85
RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization.
Meng YH, Zhou WJ, Jin LP, Liu LB, Chang KK, Mei J, Li H, Wang J, Li DJ, Li MQ
Cell death & disease 2017 Oct 12;8(10):e3105
Cell death & disease 2017 Oct 12;8(10):e3105
Micropipette force probe to quantify single-cell force generation: application to T-cell activation.
Sawicka A, Babataheri A, Dogniaux S, Barakat AI, Gonzalez-Rodriguez D, Hivroz C, Husson J
Molecular biology of the cell 2017 Nov 7;28(23):3229-3239
Molecular biology of the cell 2017 Nov 7;28(23):3229-3239
Pooled CRISPR screening with single-cell transcriptome readout.
Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C
Nature methods 2017 Mar;14(3):297-301
Nature methods 2017 Mar;14(3):297-301
Serum Gp96 is a chaperone of complement-C3 during graft-versus-host disease.
Seignez A, Joly AL, Chaumonnot K, Hazoumé A, Sanka M, Marcion G, Boudesco C, Hammann A, Seigneuric R, Jégo G, Ducoroy P, Delarue P, Senet P, Castilla-Llorente C, Solary E, Durey MA, Rubio MT, Hermine O, Kohli E, Garrido C
JCI insight 2017 Mar 23;2(6):e90531
JCI insight 2017 Mar 23;2(6):e90531
Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity.
Saitakis M, Dogniaux S, Goudot C, Bufi N, Asnacios S, Maurin M, Randriamampita C, Asnacios A, Hivroz C
eLife 2017 Jun 8;6
eLife 2017 Jun 8;6
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.
Zheng C, Zheng L, Yoo JK, Guo H, Zhang Y, Guo X, Kang B, Hu R, Huang JY, Zhang Q, Liu Z, Dong M, Hu X, Ouyang W, Peng J, Zhang Z
Cell 2017 Jun 15;169(7):1342-1356.e16
Cell 2017 Jun 15;169(7):1342-1356.e16
Predominance of weakly cytotoxic, T-bet(Low)Eomes(Neg) CD8(+) T-cells in human gastrointestinal mucosa: implications for HIV infection.
Kiniry BE, Ganesh A, Critchfield JW, Hunt PW, Hecht FM, Somsouk M, Deeks SG, Shacklett BL
Mucosal immunology 2017 Jul;10(4):1008-1020
Mucosal immunology 2017 Jul;10(4):1008-1020
Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.
Tello-Lafoz M, Martínez-Martínez G, Rodríguez-Rodríguez C, Albar JP, Huse M, Gharbi S, Merida I
Traffic (Copenhagen, Denmark) 2017 Aug;18(8):491-504
Traffic (Copenhagen, Denmark) 2017 Aug;18(8):491-504
Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.
Horng S, Therattil A, Moyon S, Gordon A, Kim K, Argaw AT, Hara Y, Mariani JN, Sawai S, Flodby P, Crandall ED, Borok Z, Sofroniew MV, Chapouly C, John GR
The Journal of clinical investigation 2017 Aug 1;127(8):3136-3151
The Journal of clinical investigation 2017 Aug 1;127(8):3136-3151
Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity.
Carcamo-Orive I, Hoffman GE, Cundiff P, Beckmann ND, D'Souza SL, Knowles JW, Patel A, Papatsenko D, Abbasi F, Reaven GM, Whalen S, Lee P, Shahbazi M, Henrion MYR, Zhu K, Wang S, Roussos P, Schadt EE, Pandey G, Chang R, Quertermous T, Lemischka I
Cell stem cell 2017 Apr 6;20(4):518-532.e9
Cell stem cell 2017 Apr 6;20(4):518-532.e9
Discovery and characterization of COVA322, a clinical-stage bispecific TNF/IL-17A inhibitor for the treatment of inflammatory diseases.
Silacci M, Lembke W, Woods R, Attinger-Toller I, Baenziger-Tobler N, Batey S, Santimaria R, von der Bey U, Koenig-Friedrich S, Zha W, Schlereth B, Locher M, Bertschinger J, Grabulovski D
mAbs 2016;8(1):141-9
mAbs 2016;8(1):141-9
Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells.
Kapoor-Kaushik N, Hinde E, Compeer EB, Yamamoto Y, Kraus F, Yang Z, Lou J, Pageon SV, Tabarin T, Gaus K, Rossy J
Frontiers in immunology 2016;7:83
Frontiers in immunology 2016;7:83
CD16(+) Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, Li X, Xu L, Shi L, Gan Y, Su Y
Frontiers in immunology 2016;7:512
Frontiers in immunology 2016;7:512
Enhanced recruitment of genetically modified CX3CR1-positive human T cells into Fractalkine/CX3CL1 expressing tumors: importance of the chemokine gradient.
Siddiqui I, Erreni M, van Brakel M, Debets R, Allavena P
Journal for immunotherapy of cancer 2016;4:21
Journal for immunotherapy of cancer 2016;4:21
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Chen J, Wang Q, Feng X, Zhang Z, Geng L, Xu T, Wang D, Sun L
Stem cells international 2016;2016:4062789
Stem cells international 2016;2016:4062789
Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy.
Du SH, Li Z, Chen C, Tan WK, Chi Z, Kwang TW, Xu XH, Wang S
PloS one 2016;11(9):e0161820
PloS one 2016;11(9):e0161820
Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types.
Momose F, Seo N, Akahori Y, Sawada S, Harada N, Ogura T, Akiyoshi K, Shiku H
PloS one 2016;11(4):e0154134
PloS one 2016;11(4):e0154134
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Son CH, Lee HR, Koh EK, Shin DY, Bae JH, Yang K, Park YS
Scientific reports 2016 Sep 27;6:32470
Scientific reports 2016 Sep 27;6:32470
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.
Pageon SV, Tabarin T, Yamamoto Y, Ma Y, Nicovich PR, Bridgeman JS, Cohnen A, Benzing C, Gao Y, Crowther MD, Tungatt K, Dolton G, Sewell AK, Price DA, Acuto O, Parton RG, Gooding JJ, Rossy J, Rossjohn J, Gaus K
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 13;113(37):E5454-63
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 13;113(37):E5454-63
Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data.
Pageon SV, Nicovich PR, Mollazade M, Tabarin T, Gaus K
Molecular biology of the cell 2016 Nov 7;27(22):3627-3636
Molecular biology of the cell 2016 Nov 7;27(22):3627-3636
T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.
Guillou L, Babataheri A, Saitakis M, Bohineust A, Dogniaux S, Hivroz C, Barakat AI, Husson J
Molecular biology of the cell 2016 Nov 7;27(22):3574-3582
Molecular biology of the cell 2016 Nov 7;27(22):3574-3582
LFA-1 Engagement Triggers T Cell Polarization at the HIV-1 Virological Synapse.
Starling S, Jolly C
Journal of virology 2016 Nov 1;90(21):9841-9854
Journal of virology 2016 Nov 1;90(21):9841-9854
Factor H uptake regulates intracellular C3 activation during apoptosis and decreases the inflammatory potential of nucleosomes.
Martin M, Leffler J, Smoląg KI, Mytych J, Björk A, Chaves LD, Alexander JJ, Quigg RJ, Blom AM
Cell death and differentiation 2016 May;23(5):903-11
Cell death and differentiation 2016 May;23(5):903-11
Obesity decreases B cell responses in young and elderly individuals.
Frasca D, Ferracci F, Diaz A, Romero M, Lechner S, Blomberg BB
Obesity (Silver Spring, Md.) 2016 Mar;24(3):615-25
Obesity (Silver Spring, Md.) 2016 Mar;24(3):615-25
Cytokine-induced killer cell therapy for the treatment of primary hepatocellular carcinoma subsequent to liver transplantation: A case report.
Li R, Yan F, Liu L, Li H, Ren B, Hui Z, Ren X
Oncology letters 2016 Mar;11(3):1885-1888
Oncology letters 2016 Mar;11(3):1885-1888
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Liu Z, Han H, He X, Li S, Wu C, Yu C, Wang S
Oncology letters 2016 Mar;11(3):1829-1834
Oncology letters 2016 Mar;11(3):1829-1834
Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.
Hu H, Wang H, Xiao Y, Jin J, Chang JH, Zou Q, Xie X, Cheng X, Sun SC
The Journal of experimental medicine 2016 Mar 7;213(3):399-414
The Journal of experimental medicine 2016 Mar 7;213(3):399-414
Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.
Soh KT, Tario JD Jr, Colligan S, Maguire O, Pan D, Minderman H, Wallace PK
Current protocols in cytometry 2016 Jan 6;75:7.45.1-7.45.33
Current protocols in cytometry 2016 Jan 6;75:7.45.1-7.45.33
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
You Y, Tan JX, Dai HS, Chen HW, Xu XJ, Yang AG, Zhang YJ, Bai LH, Bie P
Oncotarget 2016 Aug 30;7(35):57099-57116
Oncotarget 2016 Aug 30;7(35):57099-57116
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes.
Jones RB, Mueller S, O'Connor R, Rimpel K, Sloan DD, Karel D, Wong HC, Jeng EK, Thomas AS, Whitney JB, Lim SY, Kovacs C, Benko E, Karandish S, Huang SH, Buzon MJ, Lichterfeld M, Irrinki A, Murry JP, Tsai A, Yu H, Geleziunas R, Trocha A, Ostrowski MA, Irvine DJ, Walker BD
PLoS pathogens 2016 Apr;12(4):e1005545
PLoS pathogens 2016 Apr;12(4):e1005545
Effect of cytokine-induced killer cells on immune function in patients with lung cancer.
Pan Y, Wu Y, Ji J, Cai H, Wang H, Jiang Y, Sang L, Yang J, Gao Y, Liu Y, Yin L, Zhang LI
Oncology letters 2016 Apr;11(4):2827-2834
Oncology letters 2016 Apr;11(4):2827-2834
Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab.
Ramakrishna V, Sundarapandiyan K, Zhao B, Bylesjo M, Marsh HC, Keler T
Journal for immunotherapy of cancer 2015;3:37
Journal for immunotherapy of cancer 2015;3:37
RAX2: a genome-wide detection method of condition-associated transcription variation.
Tan YD, Deng J, Neilson JR
Nucleic acids research 2015 Sep 3;43(15):e96
Nucleic acids research 2015 Sep 3;43(15):e96
IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1.
Hsu P, Santner-Nanan B, Hu M, Skarratt K, Lee CH, Stormon M, Wong M, Fuller SJ, Nanan R
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3665-74
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3665-74
Soluble PD-1 aggravates progression of collagen-induced arthritis through Th1 and Th17 pathways.
Liu C, Jiang J, Gao L, Wang X, Hu X, Wu M, Wu J, Xu T, Shi Q, Zhang X
Arthritis research & therapy 2015 Nov 25;17:340
Arthritis research & therapy 2015 Nov 25;17:340
HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes.
Vassena L, Giuliani E, Koppensteiner H, Bolduan S, Schindler M, Doria M
Journal of virology 2015 May;89(10):5687-700
Journal of virology 2015 May;89(10):5687-700
Noninvasive detection of tumor-infiltrating T cells by PET reporter imaging.
McCracken MN, Vatakis DN, Dixit D, McLaughlin J, Zack JA, Witte ON
The Journal of clinical investigation 2015 May;125(5):1815-26
The Journal of clinical investigation 2015 May;125(5):1815-26
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Kim SY, Theunissen JW, Balibalos J, Liao-Chan S, Babcock MC, Wong T, Cairns B, Gonzalez D, van der Horst EH, Perez M, Levashova Z, Chinn L, D'Alessio JA, Flory M, Bermudez A, Jackson DY, Ha E, Monteon J, Bruhns MF, Chen G, Migone TS
Blood cancer journal 2015 May 29;5(5):e316
Blood cancer journal 2015 May 29;5(5):e316
Wide-scale quantitative phosphoproteomic analysis reveals that cold treatment of T cells closely mimics soluble antibody stimulation.
Ji Q, Salomon AR
Journal of proteome research 2015 May 1;14(5):2082-9
Journal of proteome research 2015 May 1;14(5):2082-9
Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.
Kumari S, Depoil D, Martinelli R, Judokusumo E, Carmona G, Gertler FB, Kam LC, Carman CV, Burkhardt JK, Irvine DJ, Dustin ML
eLife 2015 Mar 11;4
eLife 2015 Mar 11;4
Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints.
Mikucki ME, Fisher DT, Matsuzaki J, Skitzki JJ, Gaulin NB, Muhitch JB, Ku AW, Frelinger JG, Odunsi K, Gajewski TF, Luster AD, Evans SS
Nature communications 2015 Jun 25;6:7458
Nature communications 2015 Jun 25;6:7458
SRC homology 2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76) N-terminal tyrosine residues regulate a dynamic signaling equilibrium involving feedback of proximal T-cell receptor (TCR) signaling.
Ji Q, Ding Y, Salomon AR
Molecular & cellular proteomics : MCP 2015 Jan;14(1):30-40
Molecular & cellular proteomics : MCP 2015 Jan;14(1):30-40
Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.
Chauhan AK, Chen C, Moore TL, DiPaolo RJ
The Journal of biological chemistry 2015 Feb 20;290(8):5127-5140
The Journal of biological chemistry 2015 Feb 20;290(8):5127-5140
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
Roberts RA, Eitas TK, Byrne JD, Johnson BM, Short PJ, McKinnon KP, Reisdorf S, Luft JC, DeSimone JM, Ting JP
Biomaterials 2015 Dec;72:1-10
Biomaterials 2015 Dec;72:1-10
A novel human STAT3 mutation presents with autoimmunity involving Th17 hyperactivation.
Wienke J, Janssen W, Scholman R, Spits H, van Gijn M, Boes M, van Montfrans J, Moes N, de Roock S
Oncotarget 2015 Aug 21;6(24):20037-42
Oncotarget 2015 Aug 21;6(24):20037-42
Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP.
Elkord E, Abd Al Samid M, Chaudhary B
Oncotarget 2015 Aug 21;6(24):20026-36
Oncotarget 2015 Aug 21;6(24):20026-36
Collagen induces maturation of human monocyte-derived dendritic cells by signaling through osteoclast-associated receptor.
Schultz HS, Nitze LM, Zeuthen LH, Keller P, Gruhler A, Pass J, Chen J, Guo L, Fleetwood AJ, Hamilton JA, Berchtold MW, Panina S
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 1;194(7):3169-79
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 1;194(7):3169-79
Analysis of ancestral and functionally relevant CD5 variants in systemic lupus erythematosus patients.
Cenit MC, Martínez-Florensa M, Consuegra M, Bonet L, Carnero-Montoro E, Armiger N, Caballero-Baños M, Arias MT, Benitez D, Ortego-Centeno N, de Ramón E, Sabio JM, García-Hernández FJ, Tolosa C, Suárez A, González-Gay MA, Bosch E, Martín J, Lozano F
PloS one 2014;9(11):e113090
PloS one 2014;9(11):e113090
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Reichwald K, Jørgensen TZ, Tougaard P, Skov S
PloS one 2014;9(1):e85793
PloS one 2014;9(1):e85793
Cell-based measurements of mitochondrial function in human subjects.
Kang JG, Wang PY, Hwang PM
Methods in enzymology 2014;542:209-21
Methods in enzymology 2014;542:209-21
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
Journal of immunology research 2014;2014:593562
Journal of immunology research 2014;2014:593562
Immunosuppressive activity of an aqueous Viola tricolor herbal extract.
Hellinger R, Koehbach J, Fedchuk H, Sauer B, Huber R, Gruber CW, Gründemann C
Journal of ethnopharmacology 2014;151(1):299-306
Journal of ethnopharmacology 2014;151(1):299-306
Apremilast is a selective PDE4 inhibitor with regulatory effects on innate immunity.
Schafer PH, Parton A, Capone L, Cedzik D, Brady H, Evans JF, Man HW, Muller GW, Stirling DI, Chopra R
Cellular signalling 2014 Sep;26(9):2016-29
Cellular signalling 2014 Sep;26(9):2016-29
Synergistic effects of IL-4 and TNFα on the induction of B7-H1 in renal cell carcinoma cells inhibiting allogeneic T cell proliferation.
Quandt D, Jasinski-Bergner S, Müller U, Schulze B, Seliger B
Journal of translational medicine 2014 May 30;12:151
Journal of translational medicine 2014 May 30;12:151
Pan-Bcl-2 inhibitor, GX15-070 (obatoclax), decreases human T regulatory lymphocytes while preserving effector T lymphocytes: a rationale for its use in combination immunotherapy.
Kim PS, Jochems C, Grenga I, Donahue RN, Tsang KY, Gulley JL, Schlom J, Farsaci B
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2622-33
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2622-33
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
Kumaresan PR, Manuri PR, Albert ND, Maiti S, Singh H, Mi T, Roszik J, Rabinovich B, Olivares S, Krishnamurthy J, Zhang L, Najjar AM, Huls MH, Lee DA, Champlin RE, Kontoyiannis DP, Cooper LJ
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Mellergaard M, Skovbakke SL, Schneider CL, Lauridsen F, Andresen L, Jensen H, Skov S
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Hartmann N, Giese NA, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Prostaglandin E2 promotes survival of naive UCB T cells via the Wnt/β-catenin pathway and alters immune reconstitution after UCBT.
Li L, Kim HT, Nellore A, Patsoukis N, Petkova V, McDonough S, Politikos I, Nikiforow S, Soiffer R, Antin JH, Ballen K, Cutler C, Ritz J, Boussiotis VA
Blood cancer journal 2014 Jan 17;4(1):e178
Blood cancer journal 2014 Jan 17;4(1):e178
Long-lasting disease stabilization in the absence of toxicity in metastatic lung cancer patients vaccinated with an epitope derived from indoleamine 2,3 dioxygenase.
Iversen TZ, Engell-Noerregaard L, Ellebaek E, Andersen R, Larsen SK, Bjoern J, Zeyher C, Gouttefangeas C, Thomsen BM, Holm B, Thor Straten P, Mellemgaard A, Andersen MH, Svane IM
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 1;20(1):221-32
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 1;20(1):221-32
A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.
Wang D, Feng X, Lu L, Konkel JE, Zhang H, Chen Z, Li X, Gao X, Lu L, Shi S, Chen W, Sun L
Arthritis & rheumatology (Hoboken, N.J.) 2014 Aug;66(8):2234-45
Arthritis & rheumatology (Hoboken, N.J.) 2014 Aug;66(8):2234-45
Alterations in both the activatory and inhibitory potential of peripheral blood CD4+ T cells in rheumatoid arthritis patients correlate with disease progression.
Kosmaczewska A, Ciszak L, Swierkot J, Szteblich A, Wiland P, Frydecka I
Pathology oncology research : POR 2014 Apr;20(2):235-43
Pathology oncology research : POR 2014 Apr;20(2):235-43
Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.
Guo YE, Riley KJ, Iwasaki A, Steitz JA
Molecular cell 2014 Apr 10;54(1):67-79
Molecular cell 2014 Apr 10;54(1):67-79
IL-10 conditioning of human skin affects the distribution of migratory dendritic cell subsets and functional T cell differentiation.
Lindenberg JJ, Oosterhoff D, Sombroek CC, Lougheed SM, Hooijberg E, Stam AG, Santegoets SJ, Tijssen HJ, Buter J, Pinedo HM, van den Eertwegh AJ, Scheper RJ, Koenen HJ, van de Ven R, de Gruijl TD
PloS one 2013;8(7):e70237
PloS one 2013;8(7):e70237
Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.
Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, Yang G, Maiti S, Manuri P, Senyukov V, Jena B, Kebriaei P, Champlin RE, Huls H, Cooper LJ
PloS one 2013;8(5):e64138
PloS one 2013;8(5):e64138
GFP affects human T cell activation and cytokine production following in vitro stimulation.
Koelsch KA, Wang Y, Maier-Moore JS, Sawalha AH, Wren JD
PloS one 2013;8(4):e50068
PloS one 2013;8(4):e50068
An efficient low cost method for gene transfer to T lymphocytes.
Chicaybam L, Sodre AL, Curzio BA, Bonamino MH
PloS one 2013;8(3):e60298
PloS one 2013;8(3):e60298
Production and first-in-man use of T cells engineered to express a HSVTK-CD34 sort-suicide gene.
Zhan H, Gilmour K, Chan L, Farzaneh F, McNicol AM, Xu JH, Adams S, Fehse B, Veys P, Thrasher A, Gaspar H, Qasim W
PloS one 2013;8(10):e77106
PloS one 2013;8(10):e77106
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one 2013;8(10):e75297
PloS one 2013;8(10):e75297
NF-κB is activated in CD4+ iNKT cells by sickle cell disease and mediates rapid induction of adenosine A2A receptors.
Lin G, Field JJ, Yu JC, Ken R, Neuberg D, Nathan DG, Linden J
PloS one 2013;8(10):e74664
PloS one 2013;8(10):e74664
Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.
Abrahamsson SV, Angelini DF, Dubinsky AN, Morel E, Oh U, Jones JL, Carassiti D, Reynolds R, Salvetti M, Calabresi PA, Coles AJ, Battistini L, Martin R, Burt RK, Muraro PA
Brain : a journal of neurology 2013 Sep;136(Pt 9):2888-903
Brain : a journal of neurology 2013 Sep;136(Pt 9):2888-903
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Palin AC, Ramachandran V, Acharya S, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Functional characterization of EZH2β reveals the increased complexity of EZH2 isoforms involved in the regulation of mammalian gene expression.
Grzenda A, Lomberk G, Svingen P, Mathison A, Calvo E, Iovanna J, Xiong Y, Faubion W, Urrutia R
Epigenetics & chromatin 2013 Feb 28;6(1):3
Epigenetics & chromatin 2013 Feb 28;6(1):3
Thrombospondin-1 is a CD47-dependent endogenous inhibitor of hydrogen sulfide signaling in T cell activation.
Miller TW, Kaur S, Ivins-O'Keefe K, Roberts DD
Matrix biology : journal of the International Society for Matrix Biology 2013 Aug 8;32(6):316-24
Matrix biology : journal of the International Society for Matrix Biology 2013 Aug 8;32(6):316-24
The infectious synapse formed between mature dendritic cells and CD4(+) T cells is independent of the presence of the HIV-1 envelope glycoprotein.
Rodriguez-Plata MT, Puigdomènech I, Izquierdo-Useros N, Puertas MC, Carrillo J, Erkizia I, Clotet B, Blanco J, Martinez-Picado J
Retrovirology 2013 Apr 16;10:42
Retrovirology 2013 Apr 16;10:42
Functional characterization of a STAT3-dependent dendritic cell-derived CD14(+) cell population arising upon IL-10-driven maturation.
Lindenberg JJ, van de Ven R, Lougheed SM, Zomer A, Santegoets SJ, Griffioen AW, Hooijberg E, van den Eertwegh AJ, Thijssen VL, Scheper RJ, Oosterhoff D, de Gruijl TD
Oncoimmunology 2013 Apr 1;2(4):e23837
Oncoimmunology 2013 Apr 1;2(4):e23837
Ectonucleotidase CD38 demarcates regulatory, memory-like CD8+ T cells with IFN-γ-mediated suppressor activities.
Bahri R, Bollinger A, Bollinger T, Orinska Z, Bulfone-Paus S
PloS one 2012;7(9):e45234
PloS one 2012;7(9):e45234
Stimulation of Wnt/ß-catenin pathway in human CD8+ T lymphocytes from blood and lung tumors leads to a shared young/memory phenotype.
Forget MA, Huon Y, Reuben A, Grange C, Liberman M, Martin J, Mes-Masson AM, Arbour N, Lapointe R
PloS one 2012;7(7):e41074
PloS one 2012;7(7):e41074
Recombinant expression of margatoxin and agitoxin-2 in Pichia pastoris: an efficient method for production of KV1.3 channel blockers.
Anangi R, Koshy S, Huq R, Beeton C, Chuang WJ, King GF
PloS one 2012;7(12):e52965
PloS one 2012;7(12):e52965
MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway.
Li M, Cao W, Liu H, Zhang W, Liu X, Cai Z, Guo J, Wang X, Hui Z, Zhang H, Wang J, Wang L
PloS one 2012;7(11):e49841
PloS one 2012;7(11):e49841
Decrease in proportion of CD19+ CD24(hi) CD27+ B cells and impairment of their suppressive function in Graves' disease.
Zha B, Wang L, Liu X, Liu J, Chen Z, Xu J, Sheng L, Li Y, Chu Y
PloS one 2012;7(11):e49835
PloS one 2012;7(11):e49835
Quantitative phosphoproteomics reveals SLP-76 dependent regulation of PAG and Src family kinases in T cells.
Cao L, Ding Y, Hung N, Yu K, Ritz A, Raphael BJ, Salomon AR
PloS one 2012;7(10):e46725
PloS one 2012;7(10):e46725
Granulocyte macrophage colony-stimulating factor treatment of a patient in myasthenic crisis: effects on regulatory T cells.
Rowin J, Thiruppathi M, Arhebamen E, Sheng J, Prabhakar BS, Meriggioli MN
Muscle & nerve 2012 Sep;46(3):449-53
Muscle & nerve 2012 Sep;46(3):449-53
An NKp30-based chimeric antigen receptor promotes T cell effector functions and antitumor efficacy in vivo.
Zhang T, Wu MR, Sentman CL
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2290-9
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2290-9
Trauma patients' elevated tumor necrosis related apoptosis inducing ligand (TRAIL) contributes to increased T cell apoptosis.
Bandyopadhyay G, Bankey PE, Miller-Graziano CL
Clinical immunology (Orlando, Fla.) 2012 Oct;145(1):44-54
Clinical immunology (Orlando, Fla.) 2012 Oct;145(1):44-54
Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system.
Larochelle C, Cayrol R, Kebir H, Alvarez JI, Lécuyer MA, Ifergan I, Viel É, Bourbonnière L, Beauseigle D, Terouz S, Hachehouche L, Gendron S, Poirier J, Jobin C, Duquette P, Flanagan K, Yednock T, Arbour N, Prat A
Brain : a journal of neurology 2012 Oct;135(Pt 10):2906-24
Brain : a journal of neurology 2012 Oct;135(Pt 10):2906-24
A C-terminally amidated analogue of ShK is a potent and selective blocker of the voltage-gated potassium channel Kv1.3.
Pennington MW, Harunur Rashid M, Tajhya RB, Beeton C, Kuyucak S, Norton RS
FEBS letters 2012 Nov 16;586(22):3996-4001
FEBS letters 2012 Nov 16;586(22):3996-4001
IL-7 drives Th1 and Th17 cytokine production in patients with primary SS despite an increase in CD4 T cells lacking the IL-7Rα.
Bikker A, Moret FM, Kruize AA, Bijlsma JW, Lafeber FP, van Roon JA
Rheumatology (Oxford, England) 2012 Jun;51(6):996-1005
Rheumatology (Oxford, England) 2012 Jun;51(6):996-1005
Myeloid suppressor cells induced by hepatitis C virus suppress T-cell responses through the production of reactive oxygen species.
Tacke RS, Lee HC, Goh C, Courtney J, Polyak SJ, Rosen HR, Hahn YS
Hepatology (Baltimore, Md.) 2012 Feb;55(2):343-53
Hepatology (Baltimore, Md.) 2012 Feb;55(2):343-53
Hydrogen sulfide is an endogenous potentiator of T cell activation.
Miller TW, Wang EA, Gould S, Stein EV, Kaur S, Lim L, Amarnath S, Fowler DH, Roberts DD
The Journal of biological chemistry 2012 Feb 3;287(6):4211-21
The Journal of biological chemistry 2012 Feb 3;287(6):4211-21
Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease.
Ioannou M, Alissafi T, Lazaridis I, Deraos G, Matsoukas J, Gravanis A, Mastorodemos V, Plaitakis A, Sharpe A, Boumpas D, Verginis P
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1136-46
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1136-46
Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Olson BM, Jankowska-Gan E, Becker JT, Vignali DA, Burlingham WJ, McNeel DG
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Tracking immune cell proliferation and cytotoxic potential using flow cytometry.
Tario JD Jr, Muirhead KA, Pan D, Munson ME, Wallace PK
Methods in molecular biology (Clifton, N.J.) 2011;699:119-64
Methods in molecular biology (Clifton, N.J.) 2011;699:119-64
Involvement of CCR6/CCL20/IL-17 axis in NSCLC disease progression.
Kirshberg S, Izhar U, Amir G, Demma J, Vernea F, Beider K, Shlomai Z, Wald H, Zamir G, Shapira OM, Peled A, Wald O
PloS one 2011;6(9):e24856
PloS one 2011;6(9):e24856
Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12.
Magrini E, Szabò I, Doni A, Cibella J, Viola A
PloS one 2011;6(8):e22482
PloS one 2011;6(8):e22482
Choice of resident costimulatory molecule can influence cell fate in human naïve CD4+ T cell differentiation.
Williams KM, Dotson AL, Otto AR, Kohlmeier JE, Benedict SH
Cellular immunology 2011;271(2):418-27
Cellular immunology 2011;271(2):418-27
Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis.
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M
Nature communications 2011;2:334
Nature communications 2011;2:334
Anti-leukemia effect of ex vivo expanded DNT cells from AML patients: a potential novel autologous T-cell adoptive immunotherapy.
Merims S, Li X, Joe B, Dokouhaki P, Han M, Childs RW, Wang ZY, Gupta V, Minden MD, Zhang L
Leukemia 2011 Sep;25(9):1415-22
Leukemia 2011 Sep;25(9):1415-22
The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor.
Jin Z, Maiti S, Huls H, Singh H, Olivares S, Mátés L, Izsvák Z, Ivics Z, Lee DA, Champlin RE, Cooper LJ
Gene therapy 2011 Sep;18(9):849-56
Gene therapy 2011 Sep;18(9):849-56
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Landrigan A, Wong MT, Utz PJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Next-generation insights into regulatory T cells: expression profiling and FoxP3 occupancy in Human.
Birzele F, Fauti T, Stahl H, Lenter MC, Simon E, Knebel D, Weith A, Hildebrandt T, Mennerich D
Nucleic acids research 2011 Oct;39(18):7946-60
Nucleic acids research 2011 Oct;39(18):7946-60
B7-h4 expression in human melanoma: its association with patients' survival and antitumor immune response.
Quandt D, Fiedler E, Boettcher D, Marsch WCh, Seliger B
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 15;17(10):3100-11
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 May 15;17(10):3100-11
Reduced thymic output, cell cycle abnormalities, and increased apoptosis of T lymphocytes in patients with cartilage-hair hypoplasia.
de la Fuente MA, Recher M, Rider NL, Strauss KA, Morton DH, Adair M, Bonilla FA, Ochs HD, Gelfand EW, Pessach IM, Walter JE, King A, Giliani S, Pai SY, Notarangelo LD
The Journal of allergy and clinical immunology 2011 Jul;128(1):139-146
The Journal of allergy and clinical immunology 2011 Jul;128(1):139-146
Positive T cell co-stimulation by TLR7/8 ligands is dependent on the cellular environment.
Richardt-Pargmann D, Wechsler M, Krieg AM, Vollmer J, Jurk M
Immunobiology 2011 Jan-Feb;216(1-2):12-23
Immunobiology 2011 Jan-Feb;216(1-2):12-23
Hypoxia potentiates glioma-mediated immunosuppression.
Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
PloS one 2011 Jan 20;6(1):e16195
PloS one 2011 Jan 20;6(1):e16195
The vitamin D analog, TX527, promotes a human CD4+CD25highCD127low regulatory T cell profile and induces a migratory signature specific for homing to sites of inflammation.
Baeke F, Korf H, Overbergh L, Verstuyf A, Thorrez L, Van Lommel L, Waer M, Schuit F, Gysemans C, Mathieu C
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):132-42
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):132-42
Interleukin-21 and cellular activation concurrently induce potent cytotoxic function and promote antiviral activity in human CD8 T cells.
Parmigiani A, Pallin MF, Schmidtmayerova H, Lichtenheld MG, Pahwa S
Human immunology 2011 Feb;72(2):115-23
Human immunology 2011 Feb;72(2):115-23
High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses.
Pollara J, Hart L, Brewer F, Pickeral J, Packard BZ, Hoxie JA, Komoriya A, Ochsenbauer C, Kappes JC, Roederer M, Huang Y, Weinhold KJ, Tomaras GD, Haynes BF, Montefiori DC, Ferrari G
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):603-12
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):603-12
Dichotomy between factors inducing the immunosuppressive enzyme IL-4-induced gene 1 (IL4I1) in B lymphocytes and mononuclear phagocytes.
Marquet J, Lasoudris F, Cousin C, Puiffe ML, Martin-Garcia N, Baud V, Chereau F, Farcet JP, Molinier-Frenkel V, Castellano F
European journal of immunology 2010 Sep;40(9):2557-68
European journal of immunology 2010 Sep;40(9):2557-68
The role of Vδ2-negative γδ T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation.
Knight A, Madrigal AJ, Grace S, Sivakumaran J, Kottaridis P, Mackinnon S, Travers PJ, Lowdell MW
Blood 2010 Sep 23;116(12):2164-72
Blood 2010 Sep 23;116(12):2164-72
Impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets.
Hislop AD, Palendira U, Leese AM, Arkwright PD, Rohrlich PS, Tangye SG, Gaspar HB, Lankester AC, Moretta A, Rickinson AB
Blood 2010 Oct 28;116(17):3249-57
Blood 2010 Oct 28;116(17):3249-57
Glioma cancer stem cells induce immunosuppressive macrophages/microglia.
Wu A, Wei J, Kong LY, Wang Y, Priebe W, Qiao W, Sawaya R, Heimberger AB
Neuro-oncology 2010 Nov;12(11):1113-25
Neuro-oncology 2010 Nov;12(11):1113-25
T-cell receptor early signalling complex activation in response to interferon-alpha receptor stimulation.
Stevens CN, Simeone AM, John S, Ahmed Z, Lucherini OM, Baldari CT, Ladbury JE
The Biochemical journal 2010 May 27;428(3):429-37
The Biochemical journal 2010 May 27;428(3):429-37
MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1.
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, Li J, Zhou H, Tang Y, Shen N
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):6773-81
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):6773-81
Glioblastoma cancer-initiating cells inhibit T-cell proliferation and effector responses by the signal transducers and activators of transcription 3 pathway.
Wei J, Barr J, Kong LY, Wang Y, Wu A, Sharma AK, Gumin J, Henry V, Colman H, Priebe W, Sawaya R, Lang FF, Heimberger AB
Molecular cancer therapeutics 2010 Jan;9(1):67-78
Molecular cancer therapeutics 2010 Jan;9(1):67-78
The oncogenic BRAF kinase inhibitor PLX4032/RG7204 does not affect the viability or function of human lymphocytes across a wide range of concentrations.
Comin-Anduix B, Chodon T, Sazegar H, Matsunaga D, Mock S, Jalil J, Escuin-Ordinas H, Chmielowski B, Koya RC, Ribas A
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Dec 15;16(24):6040-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Dec 15;16(24):6040-8
miR-155 inhibition sensitizes CD4+ Th cells for TREG mediated suppression.
Stahl HF, Fauti T, Ullrich N, Bopp T, Kubach J, Rust W, Labhart P, Alexiadis V, Becker C, Hafner M, Weith A, Lenter MC, Jonuleit H, Schmitt E, Mennerich D
PloS one 2009 Sep 24;4(9):e7158
PloS one 2009 Sep 24;4(9):e7158
A new approach for quantitative phosphoproteomic dissection of signaling pathways applied to T cell receptor activation.
Nguyen V, Cao L, Lin JT, Hung N, Ritz A, Yu K, Jianu R, Ulin SP, Raphael BJ, Laidlaw DH, Brossay L, Salomon AR
Molecular & cellular proteomics : MCP 2009 Nov;8(11):2418-31
Molecular & cellular proteomics : MCP 2009 Nov;8(11):2418-31
Highly active microbial phosphoantigen induces rapid yet sustained MEK/Erk- and PI-3K/Akt-mediated signal transduction in anti-tumor human gammadelta T-cells.
Correia DV, d'Orey F, Cardoso BA, Lança T, Grosso AR, deBarros A, Martins LR, Barata JT, Silva-Santos B
PloS one 2009 May 21;4(5):e5657
PloS one 2009 May 21;4(5):e5657
Auf1/Hnrnpd-deficient mice develop pruritic inflammatory skin disease.
Sadri N, Schneider RJ
The Journal of investigative dermatology 2009 Mar;129(3):657-70
The Journal of investigative dermatology 2009 Mar;129(3):657-70
Absence of leucine zipper in the natural FOXP3Delta2Delta7 isoform does not affect dimerization but abrogates suppressive capacity.
Mailer RK, Falk K, Rötzschke O
PloS one 2009 Jul 1;4(7):e6104
PloS one 2009 Jul 1;4(7):e6104
Clinical heterogeneity can hamper the diagnosis of patients with ZAP70 deficiency.
Turul T, Tezcan I, Artac H, de Bruin-Versteeg S, Barendregt BH, Reisli I, Sanal O, van Dongen JJ, van der Burg M
European journal of pediatrics 2009 Jan;168(1):87-93
European journal of pediatrics 2009 Jan;168(1):87-93
Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.
Lal G, Zhang N, van der Touw W, Ding Y, Ju W, Bottinger EP, Reid SP, Levy DE, Bromberg JS
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):259-73
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):259-73
Human activated T lymphocytes modulate IDO expression in tumors through Th1/Th2 balance.
Godin-Ethier J, Pelletier S, Hanafi LA, Gannon PO, Forget MA, Routy JP, Boulassel MR, Krzemien U, Tanguay S, Lattouf JB, Arbour N, Lapointe R
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7752-60
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7752-60
Identification of heme oxygenase-1-specific regulatory CD8+ T cells in cancer patients.
Andersen MH, Sørensen RB, Brimnes MK, Svane IM, Becker JC, thor Straten P
The Journal of clinical investigation 2009 Aug;119(8):2245-56
The Journal of clinical investigation 2009 Aug;119(8):2245-56
The induction of APC with a distinct tolerogenic phenotype via contact-dependent STAT3 activation.
Gur-Wahnon D, Borovsky Z, Liebergall M, Rachmilewitz J
PloS one 2009 Aug 31;4(8):e6846
PloS one 2009 Aug 31;4(8):e6846
Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases.
Puhar A, Dal Molin F, Horvath S, Ladant D, Montecucco C
PloS one 2008;3(10):e3564
PloS one 2008;3(10):e3564
The effect of artificial antigen-presenting cells with preclustered anti-CD28/-CD3/-LFA-1 monoclonal antibodies on the induction of ex vivo expansion of functional human antitumor T cells.
Zappasodi R, Di Nicola M, Carlo-Stella C, Mortarini R, Molla A, Vegetti C, Albani S, Anichini A, Gianni AM
Haematologica 2008 Oct;93(10):1523-34
Haematologica 2008 Oct;93(10):1523-34
Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-kappaB activation.
Zeng H, Di L, Fu G, Chen Y, Gao X, Xu L, Lin X, Wen R
Molecular and cellular biology 2007 Jul;27(14):5235-45
Molecular and cellular biology 2007 Jul;27(14):5235-45
Structure, inhibitor, and regulatory mechanism of Lyp, a lymphoid-specific tyrosine phosphatase implicated in autoimmune diseases.
Yu X, Sun JP, He Y, Guo X, Liu S, Zhou B, Hudmon A, Zhang ZY
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19767-72
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19767-72
Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands.
Chin LT, Chu C, Chen HM, Hsu SC, Weng BC, Chu CH
BMC biotechnology 2007 Aug 23;7:51
BMC biotechnology 2007 Aug 23;7:51
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- Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG2a K Isotype Control Purified (Product # 16-4724-85) (open histogram) or Anti-Human CD3 Functional Grade Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85).Cells in the lymphocyte gate were used for analysis.
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- Figure 9 IL-6 produced by PBLs depleted for CD4 + , CD8 + , HLA-DR + or CD56 + /CD16 + cells. (A) Freshly purified PBLs stained for CD4, CD8, CD3, HLA-DR, CD16, CD56. Depletion controls for PBLs depleted for CD4 + , CD8 + , HLA-DR + or CD16 + /CD56 + cells. PBLs and depletions were stimulated for 7 days with combinations of IL-12 (4 ng/mL), IL-15 (10 ng/mL), IL-18 (40 ng/mL) and TL1A (100 ng/mL) and stained for CD4, CD8, CD3, HLA-DR, CD16 and CD56 expression. (B) IL-6 production after 7 days by PBLs and depletions stimulated with IL-12, IL-15, IL-18 and TL1A as described above. Error bars represent the SEM of two measurements. Statistically significant differences by t-test: *** = p
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- Figure 2. F-actin foci co-localize with TCR MC and not ICAM1. ( A ) Freshly isolated mouse AND CD4 T cells were incubated with lipid bilayer reconstituted with Alexa568 tagged anti-CD3 (TCR, red) and Alexa647-ICAM1 (blue), for 2 min at 37degC. Post incubation, cells were fixed and stained for F-actin using Alexa488-phalloidin (green), and imaged using TIRF microscopy. The region marked 1 in the 'merge' panel is magnified to clearly show the co-localization of actin foci with TCR-containing MCs. Lower panels: AND mouse CD4 T cell blasts exhibit F-actin enrichment at TCR MCs sites. AND mouse CD4 T cell blasts were labeled with Alexa568-H57 Fab (TCR), and incubated with bilayer reconstituted with MHCp and Alexa405-ICAM1 for 2 min at 37degC, fixed and stained for F-actin. Region marked 2 in the 'merge' image is further magnified to show the overlap between TCR and F-actin (arrows). The insets 1 and 2 in both MHC-activated and anti-CD3-activated mouse T cells are contrasted differently from the original 'merge' image to highlight the TCR and actin distribution. ( B ) Quantitation of the fraction of TCR or ICAM1 localized with F-actin foci. AND CD4 T blasts were incubated with antigen containing bilayer for 2 min, as described above and the images acquired were processed for colocalization assessment as described in 'Materials and methods' section. Each point represents fraction of total synaptic TCR or ICAM1 associated with F-actin foci in a
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- Figure 7 UC-MSCs transferred mitochondria to activate T cells. PBMCs from SLE patients were labeled with carboxyfluorescein succinimidyl amino ester (CFSE) and treated with anti-CD3/CD28 antibodies for two days. Then they were cocultured with UC-MSCs for 12 h, which had been prelabeled with respiratory mitochondrion specific probe Mitotracker Deep Red (MDR). PBMCs were cultured with UC-MSCs through transwell as control. Then PBMCs were stained with anti-CD3, anti-CD4, or anti-CD8 dye and detected for MDR fluorescence with flow cytometry. Fluorescence microscopy was carried out similarly with anti-CD3/28 stimulation for 12 h and then cocultured for 6 h. All experiments were performed in triplicate. (a) T cells (CD3 positive) rather than non-T cells (CD3 negative) got MDR staining. (b-d) T cells cultured with UC-MSCs directly rather than through transwell got transferred mitochondria ( n = 3). Arrows indicated transferred mitochondria within lymphocytes. * p < 0.05.
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- Figure 2 TL1A induces IL-6 and TNF-alpha. Freshly purified PBMCs were incubated with IL-12 (2 ng/mL), IL-15 (10 ng/mL), IL-18 (10 ng/mL), TL1A (100 ng/mL) and TL1AAb (1 ug/mL, blocking antibody). Extra IL-15 (2 ng/mL) was added on day 3. ( A ) After 6 days, supernatants were collected and different cytokines were measured by bead-based ELISA. Error bars represent the SEM of eight measurements. Statistically significant differences are indicated by ***(t-test, P
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- Figure 1--figure supplement 3. WASP silencing causes a reduction in F-actin foci. Mouse CD4 T cell blasts were electroporated with WASP siRNAs, or with non-targeting sequences (control). The extent of depletion of protein levels was estimated using western blots. ~40% of protein depletion was achieved using WASP siRNAs (relative densitometry value mentioned on top of gel lanes). Cells electroporated with WASP siRNA exhibited significantly fewer F-actin foci. AND T cell blasts electroporated with siRNA sequences, as described above, were incubated with bilayer containing anti-CD3 and ICAM1 for 2 min, followed with fixation and staining with Alexa488-phalloidin (left panels), and imaged using TIRF microscopy. The graphs show quantitation of total F-actin (right graph) or F-actin foci (left graph) per cell, in control or WASP siRNA-treated cells. n1 = 35, n2 = 60, p = 0.238 (right) , p = 0.0025 (left graph). DOI: http://dx.doi.org/
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- Figure 2--figure supplement 4. Lack of F-actin foci in the cSMAC of primary T cells and the Jurkat T cell line. AND CD4 T cell blasts were either incubated with bilayers containing Alexa568 tagged anti-CD3 and ICAM1 (far left panels) or were labeled with Alexa568-H57 Fab and incubated with MHCp/ICAM1 bilayers (center left panels), for 2 min. Cells were fixed and stained for F-actin using Alexa488-phalloidin (middle row, green) and visualized using spinning disc confocal microscopy. Each image is a maximum intensity projection of the bottom three planes that show high intensity signals from the bilayer. Note that TCR MCs (bottom row, red) in the F-actin depleted central zone of the cell (asterisk) exhibit no significant co-localization with actin foci. This phenomenon where central microclusters lack F-actin foci was observed in >90% cells exhibiting well-defined cSMAC (number of experiments >3). F-actin foci were not detected in Jurkat T cells (center right panel, and far right insets). Jurkat T cells were activated on bilayer containing Aexa568 tagged anti-CD3 (TCR, red, bottom row) and ICAM1 for 2 min and were fixed and stained with Alexa488-phalloidin (Actin, green, middle row). As highlighted in insets, visible F-actin enrichment is missing from the TCR MCs in these cells. Scale bars, 5 um (AND T cell) and 10 um (Jurkat T cell). DOI: http://dx.doi.org/
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- Figure 6--figure supplement 2. Effect of CK666 on TCR-induced Zap70 and PLCgamma phosphorylation in human T cells. Freshly purified human CD4 T cells were treated with DMSO (control) or CK666, and incubated with bilayer reconstituted with anti-CD3 and ICAM1 for 2 min, in presence of the inhibitor. Cells were then fixed and stained with Alexa488 phalloidin (Actin, green) and either of anti-phospho Zap70 (upper panels) or anti-phospho-PLCgamma1 (Lower panels) antibodies, and subsequently imaged using TIRFM. The graph represents phospho-Zap70 (upper graph) or phospho-PLCgamma1 levels at synapse, normalized to the mean value in control cells. For upper graph, n1 = 49 n2 = 47, p = 0.976; for the lower graph, n1 = 78, n2 = 67, p = 0.004. DOI: http://dx.doi.org/
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- Figure 6--figure supplement 1. CK666 treatment leads to loss of foci-associated PLCgamma1 and phospho-HS1 from the synapse. ( A ) Images from Figure 4E were analyzed for phospho-PLCgamma1 and F-actin foci chance level co-localization, after pixel shifts of phospho-PLCgamma1 images by 5 pixels, as performed earlier, in Figure 3--figure supplement 1 . Note that both in DMSO (control) as well as CK666 treated cells, a shift of 5 pixels (x and y) in phospho-PLCgamma1 image results in significant reduction in co-localization, indicating that loss of overlap in CK666 treatment is not just due to reduction in total phospho-PLCgamma1 levels. ( B ) Characterization of the effect of CK666 vs Cytochalasin D (CytoD) on total synaptic F-actin, F-actin foci, phospho-Zap70 and phospho-PLCgamma1 levels. Mouse CD4 T cells were treated with 100 uM CK666 or 5 uM CytoD for indicated time duration, were activated on anti-CD3 and ICAM1 containing surface for 2 min in the presence of inhibitors, fixed and processed for immunofluorescence of the indicated molecules. Note that, while both CK666 as well as CytoD treatments lead to loss of F-actin foci, CytoD treatment causes a greater reduction in total F-actin and phospho-Zap70 levels. For the left graph, for total actin and F-actin foci, n1 = 123, n2 = 118, n3 = 100, n4 = 87; for phospho-Zap70, n1 = 49, n2 = 47, n3 = 53, n4 = 42; for phospho-PLCgamma1, n1 = 78, n2 = 67, n3 = 48, n4 = 42. For total phospho-Zap70, p > 0.90 , and for the rest of the da
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- Figure 6. Arp2/3 inhibition leads to defective TCR-distal signaling. ( A ) Formation of TCR MCs or CD45 exclusion does not require F-actin foci. AND CD4 T cell blasts labeled with Alexa568-H57 Fab (to assess TCR clustering), or Alexa488-CD45 Fab (for CD45 exclusion) at 40C, were then incubated with bilayers containing ICAM1 and MHCp for 2 min, fixed (to assess TCR clustering) or visualized live (for CD45 exclusion) using TIRF microscopy. The images were processed to assess TCR clustering, or CD45 and TCR colocalization using rank filter based filtering, as described in 'Materials and methods' section. For the bars showing TCR cluster intensities per cell, n1 = 45, n2 = 28, p = 0.6; for CD45 co-localization, n1 = 26, n2 = 13, p = 0.09 . ( B ) Phosphorylation of TCR-proximal molecule Zap70 is not reduced in the cells treated with CK666. T cells were treated with DMSO or CK666 for 10 min, then incubated with surface containing ICAM1/anti-CD3 for 3 min, and processed for Y319-phospho-Zap70 and imaged using TIRF. The images were quantified to obtain the synaptic levels phospho-Zap70, and plotted as normalized to mean value of the 'control' cells. In the graph shown here, n1 = 34, n2 = 38, p = 0.16 . ( C ) Synaptic phospho-PLCgamma1 levels are reduced in cells lacking F-actin foci. DMSO (control, top panel) or CK666 (bottom panel) treated AND CD4 T cell blasts were incubated with bilayer containing anti-CD3 and ICAM1 for 2 min, fixed and stained with Alexa488-ph
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- FIGURE 7: Evolution of lymphoblast membrane surface area during transendothelial migration and cell spreading. (A) Time lapse of a lymphoblast transmigrating between human aortic endothelial cells. Scale bar, 20 mum. Images were taken every 15 s. The projected surface area S proj is represented in yellow before (leftmost image) and after (rightmost image) transendothelial migration. The pore diameter, D pore , is estimated by taking the image in which the lymphoblast width is identical above and below the pore (yellow arrows). (B) D pore during transendothelial migration as a function of the lymphoblast's projected diameter before transendothelial migration (computed using D 0 = S proj /2pi, and D 0 is an equivalent diameter for a sphere whose projected area is S proj ). (C) Histogram of the duration of transmigration. The mean duration is 3 +- 2 min (mean +- SD). (D) Scanning electron microscopy images of lymphoblasts spreading on a substrate coated with anti-CD3 plus anti-CD28 activating antibodies. Scale bars, 100 mum, 20 mum, 10 mum (left to right). Yellow arrows indicate spread cells. (E) Boxplots of the apparent membrane surface area of T-lymphocytes under both passive (white-filled box) and active (blue-filled box) deformations. The bottom and top of the box indicate the 25th and 75th percentiles, respectively. Red plus signs indicate outliers. From left to right, resting T-lymphocytes initially (column 1, A 0 , n = 14) and at rupture (column 2, A *, n = 14) aspir
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- Figure 3. Relationship of F-actin foci to TCR engagement and TCR proximal signaling. ( A ) AND mouse CD4 T cell blasts were incubated with lipid bilayer containing Alexa405-ICAM1 and MHCp, or bilayer containing Alexa405-ICAM1 alone (ICAM1) at 37degC for 2 min. The cells were fixed and stained with Alexa488-phalloidin (Actin) and imaged using TIRF microscopy. The images in the right panel are reflection (interference reflection microscopy - IRM) images in the two conditions, showing cell-bilayer contact area. Actin images were further high-pass filtered using a rank-filter based subtraction method (see 'Materials and methods') to reveal spatially localized actin features within each cell, under both incubation conditions. The graph (top right) shows average intensity of actin features (F-actin foci) per cell. n1 = 31, n2 = 53, p < 0.0001 . The bottom graph shows foci induction in human CD4 T cells. Primary human CD4 T cells were incubated with bilayer containing Alexa405-ICAM1 alone, or both Alexa405-ICAM1 and Alexa568 tagged anti-CD3 at 37degC for 2 min, and were then fixed and stained for F-actin using Alexa488-phalloidin, for imaging using TIRF microscopy. The graph shows integrated intensity of actin spots per cell, each point on the graph represents a single cell. n1 = 117, n = 79, p < 0.0001 ( B ) Formation of TCR MC associated F-actin foci requires SFK signaling. AND T cell blasts were treated with PP2 for 10 min at 37degC and were labeled with Alexa568-H57 Fab. This
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- Figure 5. F-actin foci require activity of the Arp2/3 complex. ( A ) Arp2/3 complex is localized at TCR MCs. DMSO (Control, top) or CK666 treated-(bottom) AND mouse T cell blasts were labeled with Alexa568-H57 Fab and were incubated with bilayer containing ICAM1 and MHCp for 2 min at 37degC, and then fixed and immunostained with anti-Arp3 antibody. The TIRF images show relative distribution of Arp3 (left, green) and TCR (middle, red) in the TIRF plane. Note that while Arp3 distribution overlaps with MCs in control cells, MCs are significantly devoid of Arp3 in CK666-treated cells (areas from 'merge' panels further magnified in insets). The graph (far right) shows the fraction co-localization of total synaptic TCR with Arp3. n1 = 54, n2 = 41, p < 0.0001 . In this experiment, the mean intensity of Arp3 in synapse was 3.46 (+-0.022) x 10 6 for control cells, and 2.38 (+-0.015) x 10 6 for CK666 treated cells (data not shown), and it was repeated twice with similar results. ( B ) TCR MCs associated F-actin enrichment is generated by Arp2/3 complex activation. AND T cell blasts were treated with DMSO alone (control), or 100 muM CK666, or 100 muM blebbistatin for 10 min at 37degC, were labeled with Alexa568-H57 Fab (TCR, red), and incubated with Alexa405-ICAM1 (ICAM1, blue) and MHCp-containing bilayer in the presence of the specific inhibitors at 37degC for 2 min. Cells were subsequently fixed, stained with Alexa488-phalloidin and imaged using TIRF microscopy. Note that
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- Figure 7--figure supplement 1. ILP F-actin and signaling in T-EC immunological synapse is dependent on Arp2/3 activity. ( A ) Visualization of loss of F-actin in ILPs following CK666 treatment. T cells were incubated with membrane-YFP (green) expressing endothelial cells in the presence of DMSO (control, left) or CK666 (right) for 5 min, then fixed, permeabilized and stained with Alexa594-phalloidin ('Actin', pink). Note that F-actin rich protrusions are missing at the CK666-treated T cell interface. Scale bar, 5 um. ( B ) Loss of ILPs correlated with reduced phospho-HS1 at the T cell-endothelial cell synaptic interface. T cells were incubated with endothelial cell layer, as described in 'Materials and methods' for 5 min and were then fixed and stained with Alexa594-phalloidin ('Actin', green), phospho-HS1 (red) and anti-CD3 (blue). Cells were then imaged using confocal microscopy. The insets show single T cells marked in the 'Merge' image. Scale bar, 10 um. ( C ) The intensity of the indicated proteins was then analyzed in the synaptic plane without and with CK666 treatment. n1 = 58, n2 = 71, p1 = 0.031, p2, p3 < 0.0001 . DOI: http://dx.doi.org/
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- Figure 4 PEG-S-Fab can bind CEA on tumor cells and CD3 + on T cells. Notes: Flow cytometry analysis of PEG-S-Fab and S-Fab on CEA-positive LS174T cells ( A ), CEA-negative SKOV3 cells ( B ), and CD3 + T cells ( C ). The positive control anti-CD3 antibody OKT3 was used for T-cell flow cytometry. Confocal microscopy of immunofluorescence staining as described in the ""Materials and methods"" section. S-Fab ( D ) and PEG-S-Fab ( E ) on LS174T cells (upper panel) and SKOV3 cells (lower panel), respectively. The scale bar represents 30 mum. AF488, stained by the goat antihuman IgG (H + L)-AlexaFluor 488 antibody; LS174T, human colorectal cell line LS174T; SKOV3, human ovarian cancer cell line SKOV3. Abbreviations: CEA, carcinoembryonic antigen; DAPI, 4',6-diamidino-2-phenylindole; IgG, immunoglobulin G; PEG, polyethylene glycol; PEG-S-Fab, PEGylated S-Fab; S-Fab, single-domain antibody-linked Fab; FITC, fluoresceine isothiocyanate.
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- Figure 4 The expression of galectin-1 in HSCs promotes HSC-induced T cell apoptosis and Th1/Th2 cytokine balance skewing A. Flow cytometry (annexin V-FITC apoptosis detection) analyses to detect T cell apoptosis in CD3+ T cells, cultured alone or co-cultured with HSCs subjected to different pre-treatments (cell transfection for galectin-1 knockdown and overexpression: sh-3 group versus Scr group; Over group versus pcDNA3.1 group) for 48 hours at a ratio of 10:1 (T:HSC), B. ELISA showing the levels of cytokines (IFN-gamma and IL-10) in the supernatant. Data are shown as the means (+- SD) of triplicates (n = 7). *P < 0.05. NC, negative control group; Scr, non-targeting scrambled sequence group; sh, small hairpin RNA sequence transfection group; pcDNA3.1, negative control group; Over, galectin-1 overexpression group; No HSCs, T cells cultured alone.
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- Figure 2 Cell surface expression of SAIL in CLL, AML and MM patient samples and normal BMMC and PBMC controls. ( a ) Three CLL specimens analyzed by flow cytometry. CLL cells were identified as CD19/CD5 double-positive cells. The histograms present SAIL (filled) and isotype control (open) staining in the live-cell and the CLL population. ( b ) Flow cytometry analysis of three AML specimens. SAIL expression is assessed in live-cells, CD33-positive and CD34-positive cells. ( c ) Flow cytometry analysis of three MM specimens. CD38 high cells with CD56 expression were gated for MM cells. SAIL expression is assessed in the live-cell and the MM population. ( d and e ) Flow cytometry analysis of SAIL expression in BMMC ( d ) and PBMC ( e ) via co-staining with CD19, CD3, CD14, CD56, CD33, CD34 and a cocktail of lineage (LN) markers. Numbers in histograms are median-fluorescence-intensity fold-change values relative to the isotype control. Three and two representative examples are shown for the tumor and normal samples, respectively.
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- Figure 6 CD16 + monocytes promoted T-cell proliferation in SLE . CFSE-labeled CD4 + T cells isolated from freshly collected SLE blood or blood bank collected HC blood buffy coat were cocultured with CD16 + and CD16 - monocytes for 60 h in the presence of anti-CD3 (1 mug/mL) and anti-CD 28 (1 mug/mL) antibodies and M-CSF (50 ng/mL). CFSE histograms depicted the number of events ( y -axis) and the fluorescence intensity ( x -axis), with proliferating cells displaying a progressive loss in fluorescence intensity following cell division, indicative of proliferating cells. (A) Representative histogram plots from a patient with SLE displayed the frequencies of CFSE low T cells in monocyte/T-cell cocultures and T cells cultured alone. (B) Percentages of CFSE low T cells in CD4 + T cells after coculture with each monocyte subset from five healthy donors and six patients with SLE. (C) The percentage increase in CFSE low T cells in cocultures of monocytes and T cells compared with CD4 + T cells cultured alone was compared between five healthy individuals and six patients with SLE. Data were expressed as mean +- SD and analyzed by non-parametric paired t test and Mann-Whitney U test. * P < 0.05, ** P < 0.01; NS, no significance.