Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
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- Product number
- 47-0036-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (SK7), APC-eFluor™ 780, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes.
- Antibody clone number
- SK7
- Concentration
- 5 µL/Test
Submitted references Multipeptide stimulated PBMCs generate T(EM)/T(CM) for adoptive cell therapy in multiple myeloma.
Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues.
Circulating CD56(bright) NK cells inversely correlate with survival of melanoma patients.
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
A transcriptionally and functionally distinct PD-1(+) CD8(+) T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.
Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.
Increased IL-15 Production and Accumulation of Highly Differentiated CD8(+) Effector/Memory T Cells in the Bone Marrow of Persons with Cytomegalovirus.
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
Prostaglandin E(2)-mediated adenosinergic effects on CD14(+) cells: Self-amplifying immunosuppression in cancer.
Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes.
Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.
Human and Murine Hematopoietic Stem Cell Aging Is Associated with Functional Impairments and Intrinsic Megakaryocytic/Erythroid Bias.
Platelet-Derived Ectosomes Reduce NK Cell Function.
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Switching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts.
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
A novel ex vivo isolation and expansion procedure for chimeric antigen receptor engrafted human T cells.
CALGB 150905 (Alliance): rituximab broadens the antilymphoma response by activating unlicensed NK cells.
KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity.
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Vardam-Kaur T, Pathangey LB, McCormick DJ, Bergsagel PL, Cohen PA, Gendler SJ
Oncotarget 2021 Sep 28;12(20):2051-2067
Oncotarget 2021 Sep 28;12(20):2051-2067
Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues.
Gajdasik DW, Gaspal F, Halford EE, Fiancette R, Dutton EE, Willis C, Rückert T, Romagnani C, Gerard A, Bevington SL, MacDonald AS, Botto M, Vyse T, Withers DR
Nature communications 2020 Jul 9;11(1):3421
Nature communications 2020 Jul 9;11(1):3421
Circulating CD56(bright) NK cells inversely correlate with survival of melanoma patients.
de Jonge K, Ebering A, Nassiri S, Maby-El Hajjami H, Ouertatani-Sakouhi H, Baumgaertner P, Speiser DE
Scientific reports 2019 Mar 14;9(1):4487
Scientific reports 2019 Mar 14;9(1):4487
Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
A transcriptionally and functionally distinct PD-1(+) CD8(+) T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.
Thommen DS, Koelzer VH, Herzig P, Roller A, Trefny M, Dimeloe S, Kiialainen A, Hanhart J, Schill C, Hess C, Savic Prince S, Wiese M, Lardinois D, Ho PC, Klein C, Karanikas V, Mertz KD, Schumacher TN, Zippelius A
Nature medicine 2018 Jul;24(7):994-1004
Nature medicine 2018 Jul;24(7):994-1004
Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.
Yunusbaeva M, Valiev R, Bilalov F, Sultanova Z, Sharipova L, Yunusbayev B
Scientific reports 2018 Apr 17;8(1):6098
Scientific reports 2018 Apr 17;8(1):6098
Increased IL-15 Production and Accumulation of Highly Differentiated CD8(+) Effector/Memory T Cells in the Bone Marrow of Persons with Cytomegalovirus.
Pangrazzi L, Naismith E, Meryk A, Keller M, Jenewein B, Trieb K, Grubeck-Loebenstein B
Frontiers in immunology 2017;8:715
Frontiers in immunology 2017;8:715
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
Lecciso M, Ocadlikova D, Sangaletti S, Trabanelli S, De Marchi E, Orioli E, Pegoraro A, Portararo P, Jandus C, Bontadini A, Redavid A, Salvestrini V, Romero P, Colombo MP, Di Virgilio F, Cavo M, Adinolfi E, Curti A
Frontiers in immunology 2017;8:1918
Frontiers in immunology 2017;8:1918
Prostaglandin E(2)-mediated adenosinergic effects on CD14(+) cells: Self-amplifying immunosuppression in cancer.
Al-Taei S, Salimu J, Spary LK, Clayton A, Lester JF, Tabi Z
Oncoimmunology 2017;6(2):e1268308
Oncoimmunology 2017;6(2):e1268308
Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes.
Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A, Tabi Z
Journal of extracellular vesicles 2017;6(1):1368823
Journal of extracellular vesicles 2017;6(1):1368823
Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.
Pathangey LB, McCurry DB, Gendler SJ, Dominguez AL, Gorman JE, Pathangey G, Mihalik LA, Dang Y, Disis ML, Cohen PA
Oncotarget 2017 Feb 14;8(7):10785-10808
Oncotarget 2017 Feb 14;8(7):10785-10808
Human and Murine Hematopoietic Stem Cell Aging Is Associated with Functional Impairments and Intrinsic Megakaryocytic/Erythroid Bias.
Rundberg Nilsson A, Soneji S, Adolfsson S, Bryder D, Pronk CJ
PloS one 2016;11(7):e0158369
PloS one 2016;11(7):e0158369
Platelet-Derived Ectosomes Reduce NK Cell Function.
Sadallah S, Schmied L, Eken C, Charoudeh HN, Amicarella F, Schifferli JA
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 1;197(5):1663-71
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 1;197(5):1663-71
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Cuff AO, Robertson FP, Stegmann KA, Pallett LJ, Maini MK, Davidson BR, Male V
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Switching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts.
Cartellieri M, Feldmann A, Koristka S, Arndt C, Loff S, Ehninger A, von Bonin M, Bejestani EP, Ehninger G, Bachmann MP
Blood cancer journal 2016 Aug 12;6(8):e458
Blood cancer journal 2016 Aug 12;6(8):e458
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
Broos CE, van Nimwegen M, Kleinjan A, ten Berge B, Muskens F, in 't Veen JC, Annema JT, Lambrecht BN, Hoogsteden HC, Hendriks RW, Kool M, van den Blink B
Respiratory research 2015 Sep 16;16(1):108
Respiratory research 2015 Sep 16;16(1):108
A novel ex vivo isolation and expansion procedure for chimeric antigen receptor engrafted human T cells.
Cartellieri M, Koristka S, Arndt C, Feldmann A, Stamova S, von Bonin M, Töpfer K, Krüger T, Geib M, Michalk I, Temme A, Bornhäuser M, Lindemann D, Ehninger G, Bachmann MP
PloS one 2014;9(4):e93745
PloS one 2014;9(4):e93745
CALGB 150905 (Alliance): rituximab broadens the antilymphoma response by activating unlicensed NK cells.
Du J, Lopez-Verges S, Pitcher BN, Johnson J, Jung SH, Zhou L, Hsu K, Czuczman MS, Cheson B, Kaplan L, Lanier LL, Venstrom JM
Cancer immunology research 2014 Sep;2(9):878-89
Cancer immunology research 2014 Sep;2(9):878-89
KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity.
Terszowski G, Klein C, Stern M
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5618-24
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5618-24
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
Zhang W, Nie L, Wang Y, Wang XP, Zhao H, Dongol S, Maharjan S, Cheng L
PloS one 2013;8(6):e66286
PloS one 2013;8(6):e66286
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD3 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Circulating percentages of Th17 cells and CCR6-positive cells in peripheral blood are increased in IVD degenerated patients when compared with controls. Heparinized peripheral whole blood cells from 20 patients and 15 healthy controls were stimulated with phorbol myristate acetate (PMA), ionomycin, and monensin for 4 h and subsequently stained with fluorochrome-labeled antibodies as described in Materials and Methods. A(a) Lymphocytes were gated by flow cytometry. A(b) CD3 + T subsets were gated by flow cytometry; the plots in the inset box represent the CD3 + T cells. A(c) Representative IL-17 expression levels in the CD3 + CD8 - T subsets (CD4 + T subsets) from each group are shown. The percentages of positive cells are shown in the upper left panels. A(d) Representative surface CCR6 expression levels on the CD3 + CD8 - IL-17 + subsets from each group are shown. The percentages of positive cells are shown in the right panel. (B) The percentage of circulating Th17 cells was significantly higher in IVD degenerated patients (2.973+-0.689%) than in the control group (1.039+-0.156%; *, p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 In vitro T cell proliferation. Mononuclear cells from psoriatic patients (red curve) and healthy controls (blue curve) were labelled with 1 uM CFSE prior to culturing and incubated for 5 days alone, with 1 ug/ml PHA ( A ) or in the presence of recombinant proteins K17 ( B ), S1 ( C ), S4 ( D ), PS1 ( E ) and PK ( F ) at concentration of 10 ug/ml. After 5 days, cells were labelled with a PE-conjugated anti-CD3 antibody and 7-AAD prior to flow cytometry analysis. Gated CD3+ lymphocytes are shown on the CFSE fluorescence histograms to demonstrate the decrease in fluorescence intensity during divisions. The higher peak for both patients and healthy controls represents a larger population of non-dividing parental cells (P). The signal of interest is the smaller peak that corresponds to dividing cells (shown using arrow under F ), and such cells are only observed in patents when treated with K17 ( B ), S1 ( C ), or S4 ( D ) proteins. Figures represent the percentage of CFSE low CD3+ cells (proliferating population).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Expression of PD-L1 on human HCC PLC/PRF/5 cells. (A) Human HCC PLC/PRF/5 cells were cultured alone in the absence of GPC3-CAR T cells in RPMI 1640 medium containing 10% FBS. (B) Human HCC PLC/PRF/5 cells were cocultured with GPC3-CAR T cells at an effector:Target ratio of 1:1 for 18 h in RPMI 1640 medium containing 10% FBS. PD-L1 was determined by flow cytometry in the CD3-negative gate, and the fixable, viable stain 780 was used for discriminating live from dead cells.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Efficient disruption of PD-1 expression on the surface of GPC3-CAR T cells. PD-1 and CAR expression on the surface of T cells were detected by flow cytometry on day 3 after the re-stimulation with anti-CD3/anti-CD28 beads. UTD, untransduced T cells; WT, wild type.