48-0036-41

antibody from Invitrogen Antibodies
Targeting: CD247 CD3H, CD3Q, CD3Z
Provider product page for 48-0036-41

Antibody data

Product number
48-0036-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD3 Monoclonal Antibody (SK7), eFluor 450, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes. The antibody SK7 recognizes chimpanzee CD3. Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
SK7
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD247 protein structure - 48-0036-41 shown in red.