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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00000919-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000919-M01, RRID:AB_537536
- Product name
- Mouse Monoclonal CD3 zeta Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CD3Z - CD3Z antigen, zeta polypeptide (TiT3 complex)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of CD247 expression in transfected 293T cell line by CD3Z monoclonal antibody (M01), clone 4A12-F6.Lane 1: CD247 transfected lysate (Predicted MW: 18.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - CD3Z monoclonal antibody (M01), clone 4A12-F6 Analysis of CD3Z expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Detection limit for recombinant GST tagged CD247 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of CD247 transfected lysate using anti-CD247 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD247 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of monoclonal antibody to CD3Z on formalin-fixed paraffin-embedded human lymph node tissue. Antibody concentration 5 ug/ml.
