A700-016

antibody from Invitrogen Antibodies

Targeting: CD247 CD3H, CD3Q, CD3Z

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-016

Provider

Invitrogen Antibodies

Product name

CD3E Recombinant Rabbit Monoclonal Antibody (BL-298-5D12)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BL-298-5D12

Vial size

100 µL

Concentration

50 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (Product # A700-016 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (A700-016) and rabbit anti-CD3E antibodies (BL16031, A304-291A). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by WB in Jurkat, MOLT4, K562, SR, and HeLa lysate. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (A700-016). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E in FFPE Molt4 cells (left) and Ramos cells (right) by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (A700-016). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E in FFPE breast carcinoma by IHC.Antibody:Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (Product # A700-016 lot 2).Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P).Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E in FFPE tonsil by IHC.Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (Product # A700-016 lot 2).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (Product # A700-016 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by immunhistochemistry.Sample: FFPE section of appendix.Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (Product # A700-016 lot 1) used at 1:250.Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E (orange), CD8 alpha (red), and PD-L1 (green) in FFPE breast carcinoma by IHC-IF. Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (Product # A700-016), rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (Product # A700-044), rabbit anti-PD-L1 recombinant monoclonal [BLR020E] (Product # A700-020). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (A700-016). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 µL of rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (Product # A700-016; lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (Product # A700-016 lot 2) used for IP at 20 µL/mg lysate. CD3E was also immunoprecipitated by a previous lot of this antibody (lot A700-016-1). For blotting immunoprecipitated CD3E (Product # A700-016) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.