M00131

antibody from Boster Biological Technology

Targeting: TLR2 CD282, TIL4

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00131

Provider

Boster Biological Technology

Product name

Anti-TLR2 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for TLR2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat TLR2 information: Molecular Weight: 89838 MW; Subcellular Localization: Membrane ; Single-pass type I membrane protein ; Tissue Specificity: Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

ADH-20

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy.

Xing T, Luo D, Zhao X, Xu X, Li J, Zhang L, Gao F
Journal of the science of food and agriculture 2021 Jan 15;101(1):279-286

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Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis.

Feng S, Ju L, Shao Z, Grzanna M, Jia L, Liu M
Journal of inflammation research 2020;13:343-356

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Activation of Toll-Like Receptor 2 Promotes Proliferation of Human Lung Adenocarcinoma Cells.

Gergen AK, Kohtz PD, Halpern AL, Li A, Meng X, Reece TB, Fullerton DA, Weyant MJ
Anticancer research 2020 Oct;40(10):5361-5369

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Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway.

Jin X, Yuan Y, Zhang C, Zhou Y, Song Y, Wei Z, Zhang G
Journal of veterinary science 2020 May;21(3):e50

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Up-regulated TLR2 and TLR4 expressions in liver and spleen during acute murine T. gondii infection.

Peng J, Lin X, Lin H, Chen S, Liu J, Guo Z, Liang Y, Huang S, Lu F
Parasitology research 2016 Dec;115(12):4681-4686

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Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways.

Wang Y, Ge P, Yang L, Wu C, Zha H, Luo T, Zhu Y
Journal of neuroinflammation 2014 Jan 24;11:15

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The effect of peptidoglycan stimulation on basophil-mediated atopic responses during pregnancy and in newborns.

Yang L, Xu WG, Xu YP, Guo YS, Xiong Y, Shi GY
The Journal of asthma : official journal of the Association for the Care of Asthma 2011 May;48(4):374-9

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kidney, using TLR2 Antibody(M00131)TLR2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TLR2 Antibody (M00131)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image