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LearnNBP2-25152
antibody from Novus Biologicals
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- NBP2-25152 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Chicken Polyclonal Lamin A + C Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.
