MA1-5820

antibody from Invitrogen Antibodies

Targeting: LMNA CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-5820

Provider

Invitrogen Antibodies

Product name

Lamin A/C Monoclonal Antibody (Jol3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-5820 reacts with human dermal fibroblasts in immunocytochemistry. Other cell/tissue types have not been tested in this application. It reacts with human, mouse and rat samples in Western blot. This antibody recognizes the common tail domain of Lamin A+C, epitope aa 464-572.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

Jol3

Vial size

1 mL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Nuclear localization of histamine receptor 2 in primary human lymphatic endothelial cells.

Pal S, Gashev A, Roy D
Biology open 2022 Jul 15;11(7)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), U-2 OS (Lane 3), Jurkat (Lane 4) and HL-60 (Lane 5). The blot was probed with Anti- Lamin A/C Antibody (Product # MA1-5820, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). 74 and 65 kDa band corresponding to Lamin A and C respectively were observed across all the cell lines tested except Jurkat and HL-60.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Lamin A/C using monoclonal antibody (Product # MA1-5820).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation-Western Blot (ChIP-WB) of endogenous Lamin A/C proteins using Anti-Lamin A/C Antibody: ChIP was performed using Lamin A/C Mouse Monoclonal Antibody (Product # MA1-5820, 10 µg) (lane 2) on sheared chromatin from 4 million formaldehyde fixed HeLa cells. Normal Rabbit IgG (lane 1) was used as negative IP control. Western blot analysis of immunoprecipitated proteins was performed using Lamin A Mouse Monoclonal Antibody (Product # MA1-06101, 1 µg/mL) and Lamin C Rabbit Polyclonal Antibody (Product # PA1-5827, 1:1000 dilution).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2. Colocalization of H2R with signaling partner G alpha S and nuclear envelope marker Lamin A in the nucleus. (A.1-3) Confocal images of LECs, labeled for G alpha S (green channel, A.1), H2R (red channel, A.2) and overlay merge of G alpha S, H2R and nuclear marker (DAPI) (A.3), showing the colocalization of H2R with signaling partner G alpha S in the nucleus. (B.1-3) LECs labeled with lymphatic specific marker Prox-1 (B.1), G alpha S (B.2) and overlay (B.3), showing expression of G alpha S in LECs. (C.1-3) Confocal images of nuclear envelope marker Lamin A (C.1) and H2R (C.2), demonstrating H2R localization in the nucleus in the overlay image (C.3). Scale bar: 20 um.