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LearnPA1-5827
antibody from Invitrogen Antibodies
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA1-5827 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Lamin C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-5827 detects Lamin C in human, rat and mouse samples. PA1-5827 has been successfully used in immunofluorescence, Western blot and immunohistochemistry procedures. PA1-5827 reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kDa. The PA1-5827 immunogen is a synthetic peptide corresponding to the N-terminal residues K-HHVSGSRR of human lamin C, coupled to KLH.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- Lot Dependent
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), HEK293T (Lane 4), U-2 OS (Lane 5), Jurkat (Lane 6) and HL-60 (Lane 7). The blot was probed with Anti- Lamin C Antibody (Product # PA1-5827, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 65 kDa band corresponding to Lamin A was observed across all the cell lines tested except Jurkat and HL-60.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lamin C on formalin-fixed paraffin embedded skin tissue using Lamin C polyclonal antibody (Product # PA1-5827) at a dilution of 1:100.
