NBP2-59933
antibody from Novus Biologicals
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-59933 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lamin A + C Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.