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LearnNBP2-59937
antibody from Novus Biologicals
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-59937 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lamin A + C Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human, mouse Lamin A + C antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lamin A + C Antibody (LASS2D9) [NBP2-59937] - Analysis in whole cell lysate from human (Hela) and mouse (C2C12, mouse adult fibroblasts (MAFs)) cell lines cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with LASS2D9 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. The predicted molecular weight for lamin A is 74 kDA and for lamin C 65 kDa. The absence of a band in LMNA null MAFs indicated that the antibody specifically recognizes products of LMNA gene.
