Explore
Validate
Learn711430
antibody from Invitrogen Antibodies
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 711430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lamin A/C Recombinant Polyclonal Antibody (9HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Rabbit, Horse and Pig
- Antibody clone number
- 9HCLC
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1), Hep G2 (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-3 (Lane 5) and U-87 MG (Lane 6). The blots were probed with Anti-Lamin A/C Recombinant Rabbit Polyclonal Antibody (Product # 711430, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 74 kDa and 63 kDa band corresponding to Lamin A/C was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
