PA5-37137

antibody from Invitrogen Antibodies

Targeting: KCNJ10 Kir1.2, Kir4.1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37137

Provider

Invitrogen Antibodies

Product name

Kir4.1 (KCNJ10) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 42 kDa.

Concentration

1 mg/mL

References

Submitted references

Mechanisms of hearing loss and cell death in the cochlea of connexin mutant mice.

Chen B, Xu H, Mi Y, Jiang W, Guo D, Zhang J, Zhao Y, Tang W
American journal of physiology. Cell physiology 2020 Sep 1;319(3):C569-C578

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Kir4.1 (KCNJ10) in Lane 1: Jurkat whole cell lysate, Lane 2: mouse brain tissue lysate, Lane 3: rat brain tissue lysate. Samples were incubated with Kir4.1 (KCNJ10) polyclonal antibody (Product # PA5-37137) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KIR4.1 using KIR4.1 polyclonal antibody (Product # PA5-37137) at a dilution of 1:500. Lane 1: Jurkat whole cell lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Kir4.1 (KCNJ10) Polyclonal Antibody (Product # PA5-37137) and a 42 kDa band corresponding to KCNJ10 was observed in Mouse Kidney in comparison to Mouse Skeletal Muscle which is reported to be negative. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1) and Mouse Skeletal Muscle (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).