PA5-77822

antibody from Invitrogen Antibodies

Targeting: KCNJ10 Kir1.2, Kir4.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-77822

Provider

Invitrogen Antibodies

Product name

Kir4.1 (KCNJ10) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A 1:1,000 dilution of PA5-77822 was sufficient for detection of Kir4.1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 42 kDa.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KCNJ10 in rat liver cell lysates with 15 µg of sample. The sample was blocked with 5% skim milk in TBST, incubated with KCNJ10 polyclonal antibody (Product # PA5-77822) using a dilution of 1:1000 (2 hours at 4°C), followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1) Molecular Weight Ladder (MW), Lane 2) Rat Liver cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KCNJ10 in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KCNJ10 polyclonal antibody (Product # PA5-77822) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Kir4,1 Antibody, D) Merged image. Magnification: 60x.