APC-035-GP-25UL

antibody from Invitrogen Antibodies

Targeting: KCNJ10 Kir1.2, Kir4.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

APC-035-GP-25UL

Provider

Invitrogen Antibodies

Product name

Kir4.1 (KCNJ10) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Guinea Pig

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): - 1-3.Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:800).4-6. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody , preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Kir4.1 in human U-87 MG glioblastoma cell line - Immunocytochemical staining of fixed and permeabilized U-87 MG cells using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:100) followed by goat Anti-guinea pig-AlexaFluor-488 secondary Antibody (green). Cell nuclei were visualized using Hoechst 33342 (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Kir4.1 in human U-87 MG glioblastoma cell line - Immunocytochemical staining of fixed and permeabilized U-87 MG cells using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:100) followed by goat Anti-guinea pig-AlexaFluor-488 secondary Antibody (green). Cell nuclei were visualized using Hoechst 33342 (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Kir4.1 in rat hippocampal dentate gyrus - Immunohistochemical staining of rat frozen brain sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:300). A.Kir4.1 staining (green) reveals Kir4.1 clustersin the molecular layer (Mol). B. The same section was stained with mouse Anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C. Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like” cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.