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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
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- Product number
- PA5-75689 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.
Tan N, Hu S, Hu Z, Wu Z, Wang B
Molecular medicine reports 2020 Aug;22(2):1257-1268
Molecular medicine reports 2020 Aug;22(2):1257-1268
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A7 in Lane 1: HEK293T whole cell lysate, Lane 2: sp2/0 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with S100A7 polyclonal antibody (Product # PA5-75689) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A7 in Lane 1: HEK293T whole cell lysate, Lane 2: sp2/0 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with S100A7 polyclonal antibody (Product # PA5-75689) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. Verification of differentially expressed proteins using western blotting. (A) Representative western blots demonstrate the expression levels of LTF, DEFA1, S100A7 and S100A9 in the MV/E isolated from the CSF. Histogram data shows the densitometric semi-quantification (mean +- SD) of (B) LTF, (C) DEFA1, (D) S100A7 and (E) S100A9 expression levels. Protein densitometric semi-quantification was normalized to the density of CD9 protein. CD9 is an exosome surface marker. *P
