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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB4475 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S100A7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human S100A7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A8, A9, A10, A11, A14, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P31151- Isotype
- IgG
- Antibody clone number
- 577513
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The small molecule rhodomyrtone suppresses TNF-α and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis.
Chorachoo J, Lambert S, Furnholm T, Roberts L, Reingold L, Auepemkiate S, Voravuthikunchai SP, Johnston A
PloS one 2018;13(10):e0205340
PloS one 2018;13(10):e0205340
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- S100A7 in Human Skin. S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
