PA5-28284

antibody from Invitrogen Antibodies

Targeting: KCNMB1 hslo-beta

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28284

Provider

Invitrogen Antibodies

Product name

KCNMB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse heart.

Concentration

1 mg/mL

References

Submitted references

Western blot analysis of BK channel β1-subunit expression should be interpreted cautiously when using commercially available antibodies.

Bhattarai Y, Fernandes R, Kadrofske MM, Lockwood LR, Galligan JJ, Xu H
Physiological reports 2014 Oct 1;2(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using KCNMB1 Polyclonal Antibody (Product # PA5-28284). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. 12% SDS PAGE. KCNMB1 Polyclonal Antibody (Product # PA5-28284) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using KCNMB1 Polyclonal Antibody (Product # PA5-28284). Sample (50 µg of whole cell lysate). Lane A: mouse heart. 12% SDS PAGE. KCNMB1 Polyclonal Antibody (Product # PA5-28284) diluted at 1:10,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Representative western blot obtained using anti-BK beta 1-subunit antibodies in MA, colonic, or kidney tissues from WT and BK beta 1-KO mice, (A) Alomone Labs (APC-036), (B) Pierce (PA5-28284), and (C) Abcam (Ab3587) antibodies detected a protein band at ~28 kDa or ~38 kDa in colons or MA from both mice. The bands in Abcam sets were diminished after preincubation of the primary antibody with the competing peptide. (D) Pierce (PA1-924), (E) Santa Cruz (sc-14749), and (F) Biorbyt (orb-101774) antibodies did not detect any band at ~28 kDa or ~21 kDa in MA or colons from WT mice. (G) Alomone Labs (APC-036), Pierce (PA1-924), Abcam (Ab3587), and Biorbyt (Orb-101774) antibodies did not detect protein band at ~28 kDa, ~38 kDa, or ~21 kDa in kidneys from WT mice. (H) Pierce (PA5-28284) and (I) Santa Cruz (sc-14749) antibodies detected the protein band at ~28 kDa in kidneys from both mice. beta -actin was reblotted on each membrane after anti-BK beta 1-subunit antibody was stripped. All representative blot images from kidney are in the tissue from same WT or BK beta 1-KO mouse, and blotted with primary anti-BK beta 1-subunit antibody at 1:200. Arrows indicate the manufacturer's recommended molecular weight of BK beta 1-subunit protein.