PA1-969

antibody from Invitrogen Antibodies

Targeting: PSMC2 MSS1, Nbla10058, S7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-969

Provider

Invitrogen Antibodies

Product name

PSMC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

PA1-969 detects proteasome 19S subunit S7 from human cells. PA1-969 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate. PA1-969 antigen is recombinant human proteasome 19S subunit S7.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

PSMC2 is overexpressed in glioma and promotes proliferation and anti-apoptosis of glioma cells.

Zheng X, Wang Y, Wang D, Wan J, Qin X, Mu Z, Hu N
World journal of surgical oncology 2022 Mar 14;20(1):84

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The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis.

Yi H, Friedman JL, Ferreira PA
The Journal of biological chemistry 2007 Nov 30;282(48):34770-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of proteasome 19S subunit S7 on HeLa cell lysate using Product # PA1-969.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PSMC2 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMC2 Rabbit Polyclonal Antibody (Product # PA1-969) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of PSMC2 in glioma. A The protein levels of PSMC2 in three pairs of glioma tissues (T) and adjacent non-tumor tissues (N) measured by Western blot. B PSMC2 protein levels were quantified in glioma ( n = 30) and normal control ( n = 30) tissues. C mRNA levels of PSMC2 in 30 glioma tissues and 30 normal control tissues. D mRNA levels of PSMC2 in gliomas of different malignant grades, in which grade I, n = 5; grade II, n = 8; grade III, n = 7; and grade IV, n = 10. T, glioma tissue; N, normal control tissue (*** p < 0.001). E ROC curves of differential expression of PSMC2 gene in cancer tissues (GBM, LGG) and normal tissues in the joint analysis of TCGA and GTEX databases

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Effect of PSMC2 on apoptosis-related proteins in glioma cells. A Western blot of PSMC2 and apoptosis pathway-related proteins after transfection of PSMC2 siRNA into U343MG cells. B Statistical results of PSMC2 and apoptosis pathway-related proteins after transfection of PSMC2 siRNA into U343MG cells