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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 659002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#659002, RRID:AB_2562962
- Product name
- Purified anti-TdT
- Antibody type
- Monoclonal
- Antigen
- Partial human TdT recombinant protein (1-508aa) expressed in E. coli.
- Description
- Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 7H9A33
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of mouse thymus (lane 1) cell, Jurkat (lane 2) and Daudi (lane 3) cells using anti-TdT antibody (clone 7H9A33).
- Conjugate
- Unconjugated
