Antibody data

Product number
12-5846-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-TdT Monoclonal Antibody (19-3), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells. Applications Reported: This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 19-3 antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5521) followed by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
19-3
Vial size
25 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
DNTT protein structure - 12-5846-80 shown in red.