Antibody data

Product number
14-9739-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
TdT Monoclonal Antibody (7UNA8U6), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody 7UNA8U6 reacts with human, mouse, and rat TdT. TdT, or terminal-deoxynucleotidyl transferase, is a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role during TCR and Ig gene rearrangement and functions to increase the diversity of the lymphocyte repertoire through the addition of N-nucleotides onto the ends of newly formed P-nucleotides. After positive selection, expression of TdT becomes decreased or absent. Expression of TdT has been shown to be present in lymphoma or lymphoblastic leukemia cells. Applications Reported: This 7UNA8U6 antibody has been reported for use in western blotting and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 7UNA8U6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human and mouse tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. This 7UNA8U6 antibody has also been tested by western blot analysis of reduced cell lysate prepared from mouse and rat cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Rat
Host
Mouse
Isotype
IgG
Antibody clone number
7UNA8U6
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
DNTT protein structure - 14-9739-80 shown in red.