14-9739-82

antibody from Invitrogen Antibodies

Targeting: DNTT TDT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9739-82

Provider

Invitrogen Antibodies

Product name

TdT Monoclonal Antibody (7UNA8U6), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody 7UNA8U6 reacts with human, mouse, and rat TdT. TdT, or terminal-deoxynucleotidyl transferase, is a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role during TCR and Ig gene rearrangement and functions to increase the diversity of the lymphocyte repertoire through the addition of N-nucleotides onto the ends of newly formed P-nucleotides. After positive selection, expression of TdT becomes decreased or absent. Expression of TdT has been shown to be present in lymphoma or lymphoblastic leukemia cells.

Antibody clone number

7UNA8U6

Concentration

0.5 mg/mL

References

Submitted references

Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes.

Komori T, Okada A, Stewart V, Alt FW
Science (New York, N.Y.) 1993 Aug 27;261(5125):1171-5

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Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny.

Bogue M, Gilfillan S, Benoist C, Mathis D
Proceedings of the National Academy of Sciences of the United States of America 1992 Nov 15;89(22):11011-5

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Clinical utility of leukemia cell terminal transferase measurements.

McCaffrey R, Lillquist A, Sallan S, Cohen E, Osband M
Cancer research 1981 Nov;41(11 Pt 2):4814-20

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Immunological detection of a conserved structure for terminal deoxynucleotidyltransferase.

Bollum FJ, Chang LM
The Journal of biological chemistry 1981 Aug 25;256(16):8767-70

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TdT Monoclonal Antibody (7UNA8U6), eBioscience™ (Product # 14-9739-80, 14-9739-82) and a 58 kDa band corresponding to DNA nucleotidylexotransferase was observed across positive cell lines (Molt-4, Jurkat and Reh) and tissue (Mouse thymus); and not across negative models (A-431, Raji, Ramos and Daudi). Whole cell extracts (30 µg lysate) of Molt-4 (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), Raji (Lane 4), Reh (Lane 5), Ramos (Lane 6), Daudi (Lane 7) and tissue extract of Mouse Thymus (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human thymus using 10 µg/mL of Anti-TdT Purified followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.