Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF7178 - Provider product page
- Provider
- R&D Systems
- Product name
- Human 17 beta-HSD1/HSD17B1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human 17 beta-HSD1/HSD17B1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P14061
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human 17 beta-HSD1/HSD17B1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- 17 beta-HSD1/HSD17B1 in Human Alzheimer's Brain. 17 beta-HSD1/HSD17B1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.