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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- AF2058 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Legumain/Asparaginyl Endopeptidase Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Legumain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Legumain is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Protease activity of legumain is inhibited by an increase of cystatin E/M in the DJ-1-knockout mouse spleen, cerebrum and heart.
7,8-Dihydroxyflavone Ameliorates Cognitive Impairment by Inhibiting Expression of Tau Pathology in ApoE-Knockout Mice.
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Yamane T, Kozuka M, Yamamoto Y, Nakano Y, Nakagaki T, Ohkubo I, Ariga H
Biochemistry and biophysics reports 2017 Mar;9:187-192
Biochemistry and biophysics reports 2017 Mar;9:187-192
7,8-Dihydroxyflavone Ameliorates Cognitive Impairment by Inhibiting Expression of Tau Pathology in ApoE-Knockout Mice.
Tan Y, Nie S, Zhu W, Liu F, Guo H, Chu J, Cao XB, Jiang X, Zhang Y, Li Y
Frontiers in aging neuroscience 2016;8:287
Frontiers in aging neuroscience 2016;8:287
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Zeeuwen PL, van Vlijmen-Willems IM, Cheng T, Rodijk-Olthuis D, Hitomi K, Hara-Nishimura I, John S, Smyth N, Reinheckel T, Hendriks WJ, Schalkwijk J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3744-55
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3744-55
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC Jr, Vlasuk GP, Pittman DD
Atherosclerosis 2008 Nov;201(1):53-66
Atherosclerosis 2008 Nov;201(1):53-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Legumain/Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Legumain/Asparaginyl Endopeptidase at approximately 37 and 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
