H00000843-M02

antibody from Abnova Corporation

Targeting: CASP10 FLICE-2, MCH4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000843-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000843-M02, RRID:AB_1111708

Product name

CASP10 monoclonal antibody (M02), clone 2E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CASP10.

Antigen sequence

MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQD
VENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDL
LSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERL
LPTRQ


Isotype

IgG

Antibody clone number

2E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CASP10 monoclonal antibody (M02), clone 2E7. Western Blot analysis of CASP10 expression in U-2 OS ( Cat # L022V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CASP10 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CASP10 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol