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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- NB100-56124 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56124, RRID:AB_837830
- Product name
- Rabbit Polyclonal Caspase-10/FLICE2 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. This polyclonal antisera recognizes caspase-10 forms that contain the peptide immunogen sequence, amino acids 373-392 (ALNPEQAPTSLQDSIPAEAD) of human caspase 10, including caspase-10a, caspase-10b, and caspase-10d.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Caspase-10/FLICE2 Antibody - Unpurified [NB100-56124] - Caspase-10/FLICE2 Antibody [NB100-56124] - Caspase 10 was detected in immersion fixed paraffin-embedded sections of human spleen using this antibody at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane in splenocytes.
