AF834

antibody from R&D Systems

Targeting: CASP10 FLICE-2, MCH4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF834

Provider

R&D Systems

Product name

Human Caspase-10 (FLICE 2) Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse Caspase-10 (FLICE 2) precursor in Western blots. Sensitivity for detection of the small subunit is not known.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.

Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Caspase-10 (FLICE 2), by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Caspase-10 (FLICE 2) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF834) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase-10 (FLICE 2), at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.