11014-1-AP

antibody from Proteintech Group

Targeting: AK2

Provider product page for 11014-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11014-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11014-1-AP, RRID:AB_2305358
Product name: AK2 antibody
Antibody type: Polyclonal
Description: AK2 antibody (Cat. #11014-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

AK2 protein structure - 11014-1-AP shown in red.

Submitted references Protein S-acylation dynamics provide metabolic plasticity to acute myeloid leukemia cells.

Balasundaram N, Erdem A, Sharda A, Daniels VW, Chea PL, Leguay F, Liu Y, Keibler MA, Vidoudez C, Lane AA, Vertommen D, Casteur H, Laurent MR, Trauger SA, Stephanopoulos G, Scadden DT, van Gastel N
bioRxiv : the preprint server for biology 2026 Mar 3;

A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality.

Okutani A, Naima J, Ogihara A, Motoichi T, Ohsawa I, Ohta Y
Biophysics and physicobiology 2025;22(2):e220012

ROS-dependent localization of glycolytic enzymes to mitochondria.

Esparza-Moltó PB, Goswami AV, Bozkurt S, Münch C, Newman LE, Moyzis AG, Rojas GR, Guan D, Jones JR, Gage FH, Shadel GS
Redox biology 2025 Oct;86:103812

Andrographolide ameliorates sepsis-induced acute liver injury by attenuating endoplasmic reticulum stress through the FKBP1A-mediated NOTCH1/AK2 pathway.

He J, Huang Z, Zou R
Cell biology and toxicology 2025 Mar 7;41(1):56

All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain.

Wilkins HN, Knerler SA, Warshanna A, Colón Ortiz R, Haas K, Orsburn BC, Williams DW
ACS pharmacology & translational science 2024 Nov 8;7(11):3626-3640

Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier.

Colón Ortiz R, Knerler S, Fridman LB, Mercado A, Price AS, Rosado-Franco JJ, Wilkins H, Flores BR, Orsburn BC, Williams DW
Fluids and barriers of the CNS 2024 Jan 10;21(1):5

Cocaine Regulates Antiretroviral Therapy CNS Access Through Pregnane-X Receptor-Mediated Drug Transporter and Metabolizing Enzyme Modulation at the Blood Brain Barrier.

Fridman LB, Knerler S, Price AS, Ortiz RC, Mercado A, Wilkins H, Flores BR, Orsburn BC, Williams DW
bioRxiv : the preprint server for biology 2023 Jul 29;

The palmitoyltransferase ZDHHC21 regulates oxidative phosphorylation to induce differentiation block and stemness in AML.

Shao X, Xu A, Du W, Xu T, Huang Y, Xia Z, Wang W, Cai M, Zhang X, Zhang J, Cao J, Xu X, Yang B, He Q, Ying M
Blood 2023 Jul 27;142(4):365-381

A progeroid syndrome caused by a deep intronic variant in TAPT1 is revealed by RNA/SI-NET sequencing.

Nabavizadeh N, Bressin A, Shboul M, Moreno Traspas R, Chia PH, Bonnard C, Szenker-Ravi E, Sarıbaş B, Beillard E, Altunoglu U, Hojati Z, Drutman S, Freier S, El-Khateeb M, Fathallah R, Casanova JL, Soror W, Arafat A, Escande-Beillard N, Mayer A, Reversade B
EMBO molecular medicine 2023 Feb 8;15(2):e16478

Discovery of Hepatotoxic Equivalent Markers and Mechanism of Polygonum multiflorum Thunb. by Metabolomics Coupled with Molecular Docking.

Zhang Y, Liu L, Feng M, Wu H, Dai Y, Jia Z, Fang C, Liu M, Yan X, Zhu M, Huang B, Qu B, Xiao H
Molecules (Basel, Switzerland) 2022 Dec 21;28(1)

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Escande-Beillard N, Loh A, Saleem SN, Kanata K, Hashimoto Y, Altunoglu U, Metoska A, Grandjean J, Ng FM, Pomp O, Baburajendran N, Wong J, Hill J, Beillard E, Cozzone P, Zaki M, Kayserili H, Hamada H, Shiratori H, Reversade B
Neuron 2020 Jul 8;107(1):82-94.e6

A loss-of-function NUAK2 mutation in humans causes anencephaly due to impaired Hippo-YAP signaling.

Bonnard C, Navaratnam N, Ghosh K, Chan PW, Tan TT, Pomp O, Ng AYJ, Tohari S, Changede R, Carling D, Venkatesh B, Altunoglu U, Kayserili H, Reversade B
The Journal of experimental medicine 2020 Dec 7;217(12)

Rhythmic nucleotide synthesis in the liver: temporal segregation of metabolites.

Fustin JM, Doi M, Yamada H, Komatsu R, Shimba S, Okamura H
Cell reports 2012 Apr 19;1(4):341-9

Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2.

Pannicke U, Hönig M, Hess I, Friesen C, Holzmann K, Rump EM, Barth TF, Rojewski MT, Schulz A, Boehm T, Friedrich W, Schwarz K
Nature genetics 2009 Jan;41(1):101-5

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 11014-1-AP(AK2 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of A549 cells, using AK2 antibody 11014-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-AK2 (IP:11014-1-AP, 3ug; Detection:11014-1-AP 1:800) with mouse liver tissue lysate 12500ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas cancer using 11014-1-AP(AK2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue