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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- R32502 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AK2 Antibody / Adenylate kinase 2
- Antibody type
- Polyclonal
- Description
- This highly specific AK2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the AK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) mouse kidney and 3) human HeLa lysate with AK2 antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) mouse kidney and 3) human HeLa lysate with AK2 antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human HL60, 3) human 293T, 4) human HepG2, 5) rat heart, 6) rat liver, 7) mouse heart and 8) mouse liver tissue lysate with AK2 antibody. Predicted molecular weight ~26 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with AK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung with AK2 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse kidney with AK2 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat liver with AK2 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat liver with AK2 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
