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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-49482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylate Kinase 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Zebrafish ak2 in (Lane 1) Zebrafish lysates (20 µg), (Lane 2) ZF4 whole cell lysates (20 µg), (Lane 3) human heart lysates (20 µg), and Lane 4: mouse liver lysates (20 µg). Samples were probed with a Zebrafish ak2 Antibody (Center) (Product # PA5-49482) at 1:2000 dilution. Secondary Goat Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Zebrafish ak2 in paraformaldehyde-fixed, paraffin-embedded zebra fish body tissue sections. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with a ak2 antibody (Product # PA5-49482) at a 1:25 dilution for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Zebrafish ak2 in paraformaldehyde-fixed, paraffin-embedded human heart tissue sections. Samples were incubated with a ak2 antibody (Product # PA5-49482) at a 1:25 dilution for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ak2 in zebra fish ZF4 cells. Cells were fixed, permeabilized, and stained with a ak2 polyclonal antibody (Product # PA5-49482, green line) at a dilution of 1:25 in 2% BSA. Following incubation of the primary antibody for 1 hour at 37°C, cells were stained with a secondary Goat-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed antibody at a 1:400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
