F54506

antibody from NSJ Bioreagents

Targeting: AK2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54506

Provider

NSJ Bioreagents

Product name

Zebrafish ak2 Antibody

Antibody type

Polyclonal

Description

This highly specific Zebrafish ak2 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with zebrafish, human and mouse samples.

Reactivity

Human, Mouse, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the zebrafish ak2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) whole zebrafish, 2) zebrafish ZF4, 3) human heart and 4) mouse liver lysate with zebrafish ak2 antibody. Predicted molecular weight ~26 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE zebrafish body tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized zebrafish ZF4 cells with zebrafish ak2 antibody; Blue=isotype control, Green= ak2 antibody.