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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-28611 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylate Kinase 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (95%), Rat (94%), Zebrafish (81%), Xenopus laevis (82%), Rhesus Monkey (100%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611). Sample (30 µg of whole cell lysate). A: HeLa. 12% SDS PAGE. Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Adenylate Kinase 2 Polyclonal Antibody detects Adenylate kinase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: Adenylate kinase 2 protein stained by Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Adenylate Kinase 2 was performed in paraffin-embedded rat liver tissue using Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Adenylate Kinase 2 was performed in paraffin-embedded rat pancreas tissue using Adenylate Kinase 2 Polyclonal Antibody (Product # PA5-28611) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Adenylate kinase 2 (Product # PA5-28611) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
