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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-93203 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF1C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PPLIDPWDPG LTARDLLFRG GYRYRKRPRV VLDVTEQISR FLLDHGDVAF APLGKLMLEN FKLEGAGSRT KKKTVVSVKK LLQDLGGHQP WGCPWAYLSN RQRRFSILGG PILGTSVASH LAELLHEELV LRWEQLLLDE ACTGGALAWV PGRTPQFGQL VYPAGGAQDR LHFQEVVLTP GDNPQFLGKP GRIQLQGPVR QVVTCTVQGE TLLAVRSDYH CAVWKFGKQW QPTLLQAMQV EKGATGISLS P; Positive Samples: 293T, MCF7, Mouse thymus; Cellular Location: Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using TAF1C Polyclonal antibody (Product # PA5-93203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAF1C in extracts of various cell lines using TAF1C Polyclonal Antibody (Product # PA5-93203) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAF1C in extracts of various cell lines using TAF1C Polyclonal Antibody (Product # PA5-93203) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TAF1C in paraffin-embedded mouse lung using TAF1C Polyclonal Antibody (Product # PA5-93203) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TAF1C in paraffin-embedded human gastric cancer using TAF1C Polyclonal Antibody (Product # PA5-93203) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TAF1C in paraffin-embedded mouse lung using TAF1C Polyclonal Antibody (Product # PA5-93203) at a dilution of 1:200.
