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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX114609 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114609, RRID:AB_11178636
- Product name
- P2Y2 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE GTX114609 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (GTX114609) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- P2Y2 antibody [N3C3] detects P2Y2 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 protein stained by P2Y2 antibody [N3C3] (GTX114609) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 £gg of P2Y2 antibody [N3C3] (GTX114609).Western blot analysis was performed using P2Y2 antibody [N3C3] (GTX114609).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
