Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- APR-102-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2Y2 Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8): - 1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200).5-8. Anti-P2Y2 Receptor (extracellular) Antibody , preincubated with P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8): - 1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200).5-8. Anti-P2Y2 Receptor (extracellular) Antibody , preincubated with P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of P2Y2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. P2RY2 immunoreactivity (green) in the hippocampal dentate gyrus appears in blood vessels (horizontal arrows) and in neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of P2RY2 in live intact mouse J774 macrophage cells: - (black) cells. (red) Cells + rabbit IgG isotype control + goat- Anti-rabbit-FITC. (green) Cells + Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (5 µg) + goat- Anti-rabbit-FITC.